SAP102 antibody - 124 214 K.O.

SAP102 is a component of postsynaptic densities

Guinea pig polyclonal antiserum

Cat. No.: 124 214

Amount: 100 µl

Price: $365.00

Cat. No. 124 214	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 146 from mouse SAP102 (UniProt Id: P70175)
Reactivity	Reacts with: rat (Q62936), mouse (P70175). Other species not tested yet.
Specificity	K.O. validated
Data sheet	124_214.pdf

References for SAP102 - 124 214

Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 124 214 ICC; tested species: rat

Cat. No.: 124 214

Amount: 100 µl

Price: $365.00

ICC

Background

SAP 102 (synapse associated protein of 102 kDa, also called DLG 3) belongs to the PSD 95 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. It is a component of postsynaptic densities in central synapses. It is involved in NMDA receptor clustering and immobilization. In vitro, all three PDZ domains in SAP 102 bind the cytoplasmic tail of NR2B.

1 ) Fujita A et al., Biochem. Biophys. Res. Commun. (2000)
2 ) Gundelfinger ED et al., Naturwissenschaften (2000)
3 ) Lau LF et al., J. Biol. Chem. (1996)
4 ) Müller BM et al., Neuron (1996)

Protocols

Standard protocol

ICC

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