Synaptic Systems

Cat. No.: 294 303

Amount: 50 µg

Price: $375.00

A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 294 303 WB; tested species: rat

WB

Background

Synaptic adhesion-like molecules (SALMs) are a family of cell adhesion molecules also known as LRFN (leucine-rich repeat and fibronectin III domain-containing). They are involved in neurite outgrowth and synapse formation.
SALM 1, 2, and 3 contain a cytoplasmic C-terminal PDZ-binding motif which is not present in SALM 4 and 5.

1 ) Mah W et al., J. Neurosci. (2010)
2 ) Seabold GK et al., J. Biol. Chem. (2008)
3 ) Morimura N et al., Gene (2006)

Protocols

WB

OTHER

Preabsorbtion

Other products for this target

Cat. No. 294 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 595 to 608 from mouse Salm3 (UniProt Id: Q80XU8)
Reactivity	Reacts with: rat (Q80XU8), mouse (Q80XU8). Other species not tested yet.
Matching control protein/peptide	294-3P
Data sheet	294_303.pdf

SALM3 antibody - 294 303

References for SALM3 - 294 303