SALM2 antibody - 294 203

SALMs are neuronal adhesion proteins

Rabbit polyclonal purified antibody

Cat. No.: 294 203

Amount: 50 µg

Price: $375.00

Cat. No. 294 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 404 to 420 from rat SALM2 (UniProt Id: P0C7J6)
Reactivity	Reacts with: rat (P0C7J6), mouse (Q2WF71). Other species not tested yet.
Matching control protein/peptide	294-2P
Data sheet	294_203.pdf

Cat. No.: 294 203

Amount: 50 µg

Price: $375.00

Background

Synaptic adhesion-like molecules (SALMs) are a family of cell adhesion molecules also known as LRFN (leucine-rich repeat and fibronectin III domain-containing). They are involved in neurite outgrowth and synapse formation.
SALM 1, 2, and 3 contain a cytoplasmic C-terminal PDZ-binding motif which is not present in SALM 4 and 5.

1 ) Mah W et al., J. Neurosci. (2010)
2 ) Seabold GK et al., J. Biol. Chem. (2008)
3 ) Morimura N et al., Gene (2006)

Protocols

OTHER

Preabsorbtion

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