Synaptotagmin7 protein - 105-71P

Synaptotagmin7 is a Ca²⁺ dependent regulator of exocytosis

control protein

Cat. No.: 105-71P

Amount: 100 µg

Price: $105.00

Cat. No. 105-71P	100 µg protein, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
Immunogen	Recombinant protein corresponding to AA 46 to 133 from rat Synaptotagmin7 (UniProt Id: Q62747)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	105 173
Remarks	This control protein consists of the Strep-Tag® fusion protein (aa 46 - 133 of rat synaptotagmin 7) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	105-71p.pdf

Cat. No.: 105-71P

Amount: 100 µg

Price: $105.00

Background

Synaptotagmin7 is a proposed regulator of Ca²⁺ dependent exocytosis like neurotransmitter release. It occurs in several splicing variants which are expressed in a developmentally regulated pattern in brain. The distinct roles for the alternative splicing isoforms have not yet been determined.
Synaptotagmin7 shows Ca²⁺ dependent oligomerization via its own C2 domains leading to the formation of large linear structures which reside at the fusion site of vesicles and plasma membrane. These oligomers may be involved in the modulation of Ca²⁺ dependent exocytosis by opening or dilating fusion pores.

1 ) Fukuda M et al., Biochem. J. (2002)
2 ) Fukuda M et al., J. Biol. Chem. (2002)
3 ) Sugita S et al., Neuron (2001)
4 ) Fukuda M et al., J. Biol. Chem. (2001)
5 ) Caler EV et al., J. Exp. Med. (2001)
6 ) Fukuda M et al., J. Biol. Chem. (2000)

Protocols

OTHER

Preadsorbtion

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