Cat. No.: 105-71P
Amount: 100 µg
Price:
$105.00
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| Cat. No. 105-71P |
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use. Control proteins should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
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| Immunogen | Recombinant protein corresponding to AA 46 to 133 from rat Synaptotagmin7 (UniProt Id: Q62747) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 105 173 |
| Remarks |
This control protein consists of the Strep-Tag® fusion protein (aa 46 - 133 of rat synaptotagmin 7) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 105-71p.pdf |
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Synaptotagmin7 is a proposed regulator of Ca2+ dependent exocytosis like neurotransmitter release. It occurs in several splicing variants which are expressed in a developmentally regulated pattern in brain. The distinct roles for the alternative splicing isoforms have not yet been determined.
Synaptotagmin7 shows Ca2+ dependent oligomerization via its own C2 domains leading to the formation of large linear structures which reside at the fusion site of vesicles and plasma membrane. These oligomers may be involved in the modulation of Ca2+ dependent exocytosis by opening or dilating fusion pores.