Cat. No.: 105 053
Amount: 50 µg
Price:
$375.00
Cat. No. 105 053 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 up to 1 : 1000 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 gallery |
Immunogen | Recombinant protein corresponding to AA 50 to 108 from mouse Synaptotagmin5 (UniProt Id: Q9R0N5) (UniProt Id: Q9R0N5) |
Reactivity |
Reacts with: rat (P47861), mouse (Q9R0N5). Other species not tested yet. |
Specificity | Specific for synaptotagmin 5 (UniProt Id: Q9R0N5) sometimes referred to as synaptotagmin 9. Does not detect (UniProt Id: Q9R0N9); K.O. validated PubMed: 37432648 |
Data sheet | 105_053.pdf |
Up to now at least 17 synaptotagmins have been identified. Two different synaptotagmins were independently termed synaptotagmin5 by two research groups (see Li et al., 1995 and Craxton & Goeder, 1995). The groups refer to the respective other protein as synaptotagmin9. The synaptotagmin described by Craxton & Goeder is expressed in different tissues like kidney, heart, lung and adipose tissue but shows its highest expression level in brain, where it predominantly locates to an enriched synaptic vesicle fraction. Like other synaptotagmins it contains one transmembrane and two cytosolic C2 domains.