Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 105 043

Synaptotagmin4 antibody - 105 043 K.O.

Synaptotagmin4 is located at the Golgi compartment and LDCVs in NGF differentiated PC12 cells
Rabbit polyclonal purified antibody
Cat. No.: 105 043
Amount: 50 µg
Price: $375.00
Cat. No. 105 043 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not recommended (see remarks)
IHC: not recommended
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Reactivity Reacts with: rat (P50232), mouse (P40749).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 105-4P
Remarks

ICC: Cat. no. 105 143 is recommended.
Data sheet 105_043.pdf

References for Synaptotagmin4 - 105 043

REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.
Prestigio C, Ferrante D, Marte A, Romei A, Lignani G, Onofri F, Valente P, Benfenati F, Baldelli P
eLife (2021) 10: . 105 043 WB; tested species: mouse
Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles.
Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, et al.
Developmental cell (2018) 453: 347-361.e5. 105 043 WB, IHC; tested species: mouse
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 043 WB; tested species: rat
Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 105 043 WB, ICC; tested species: pig
Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.
Andersson SA, Olsson AH, Esguerra JL, Heimann E, Ladenvall C, Edlund A, Salehi A, Taneera J, Degerman E, Groop L, Ling C, et al.
Molecular and cellular endocrinology (2012) 3641-2: 36-45. 105 043 WB
Cell therapy modulates expression of Tax1-binding protein 1 and synaptotagmin IV in a model of optic nerve lesion.
Mesentier-Louro LA, Coronel J, Zaverucha-do-Valle C, Mencalha A, Paredes BD, Abdelhay E, Mendez-Otero R, Santiago MF
Investigative ophthalmology & visual science (2012) 538: 4720-9. 105 043 WB, IHC
Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.
Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5398-413. 105 043 WB, ICC
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
Prada I, Marchaland J, Podini P, Magrassi L, D'Alessandro R, Bezzi P, Meldolesi J
The Journal of cell biology (2011) 1933: 537-49. 105 043 WB
Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.
Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH
Neuroscience (2010) 1671: 135-42. 105 043 WB, IP
Deferoxamine-induced neurite outgrowth and synapse formation in postnatal rat dorsal root ganglion (DRG) cell cultures.
Nowicki M, Kosacka J, Spanel-Borowski K, Borlak J
European journal of cell biology (2009) 8810: 551-62. 105 043 WB
Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release.
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER
Nature neuroscience (2009) 126: 767-76. 105 043 WB, app.icc; ip
Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM
Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.
Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH
Neuroscience (2010) 1671: 135-42. 105 043 WB, IP
Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 105 043 WB, ICC; tested species: pig
Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.
Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5398-413. 105 043 WB, ICC
Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles.
Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, et al.
Developmental cell (2018) 453: 347-361.e5. 105 043 WB, IHC; tested species: mouse
Cell therapy modulates expression of Tax1-binding protein 1 and synaptotagmin IV in a model of optic nerve lesion.
Mesentier-Louro LA, Coronel J, Zaverucha-do-Valle C, Mencalha A, Paredes BD, Abdelhay E, Mendez-Otero R, Santiago MF
Investigative ophthalmology & visual science (2012) 538: 4720-9. 105 043 WB, IHC
Neuropeptide exocytosis involving synaptotagmin-4 and oxytocin in hypothalamic programming of body weight and energy balance.
Zhang G, Bai H, Zhang H, Dean C, Wu Q, Li J, Guariglia S, Meng Q, Cai D
Neuron (2011) 693: 523-35. 105 043 IHC
Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM
Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release.
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER
Nature neuroscience (2009) 126: 767-76. 105 043 WB, app.icc; ip
Cat. No.: 105 043
Amount: 50 µg
Price: $375.00
REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.
Prestigio C, Ferrante D, Marte A, Romei A, Lignani G, Onofri F, Valente P, Benfenati F, Baldelli P
eLife (2021) 10: . 105 043 WB; tested species: mouse
Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles.
Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, et al.
Developmental cell (2018) 453: 347-361.e5. 105 043 WB, IHC; tested species: mouse
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 043 WB; tested species: rat
Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 105 043 WB, ICC; tested species: pig
Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes.
Andersson SA, Olsson AH, Esguerra JL, Heimann E, Ladenvall C, Edlund A, Salehi A, Taneera J, Degerman E, Groop L, Ling C, et al.
Molecular and cellular endocrinology (2012) 3641-2: 36-45. 105 043 WB
Cell therapy modulates expression of Tax1-binding protein 1 and synaptotagmin IV in a model of optic nerve lesion.
Mesentier-Louro LA, Coronel J, Zaverucha-do-Valle C, Mencalha A, Paredes BD, Abdelhay E, Mendez-Otero R, Santiago MF
Investigative ophthalmology & visual science (2012) 538: 4720-9. 105 043 WB, IHC
Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.
Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5398-413. 105 043 WB, ICC
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
Prada I, Marchaland J, Podini P, Magrassi L, D'Alessandro R, Bezzi P, Meldolesi J
The Journal of cell biology (2011) 1933: 537-49. 105 043 WB
Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.
Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH
Neuroscience (2010) 1671: 135-42. 105 043 WB, IP
Deferoxamine-induced neurite outgrowth and synapse formation in postnatal rat dorsal root ganglion (DRG) cell cultures.
Nowicki M, Kosacka J, Spanel-Borowski K, Borlak J
European journal of cell biology (2009) 8810: 551-62. 105 043 WB
Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release.
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER
Nature neuroscience (2009) 126: 767-76. 105 043 WB, app.icc; ip
Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM
Loss of synaptotagmin IV results in a reduction in synaptic vesicles and a distortion of the Golgi structure in cultured hippocampal neurons.
Arthur CP, Dean C, Pagratis M, Chapman ER, Stowell MH
Neuroscience (2010) 1671: 135-42. 105 043 WB, IP
Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis.
Tsai PS, Brewis IA, van Maaren J, Gadella BM
PloS one (2012) 73: e32603. 105 043 WB, ICC; tested species: pig
Distinct subsets of Syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity.
Dean C, Liu H, Staudt T, Stahlberg MA, Vingill S, Bückers J, Kamin D, Engelhardt J, Jackson MB, Hell SW, Chapman ER, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5398-413. 105 043 WB, ICC
Synaptotagmin 4 Regulates Pancreatic β Cell Maturation by Modulating the Ca2+ Sensitivity of Insulin Secretion Vesicles.
Huang C, Walker EM, Dadi PK, Hu R, Xu Y, Zhang W, Sanavia T, Mun J, Liu J, Nair GG, Tan HYA, et al.
Developmental cell (2018) 453: 347-361.e5. 105 043 WB, IHC; tested species: mouse
Cell therapy modulates expression of Tax1-binding protein 1 and synaptotagmin IV in a model of optic nerve lesion.
Mesentier-Louro LA, Coronel J, Zaverucha-do-Valle C, Mencalha A, Paredes BD, Abdelhay E, Mendez-Otero R, Santiago MF
Investigative ophthalmology & visual science (2012) 538: 4720-9. 105 043 WB, IHC
Neuropeptide exocytosis involving synaptotagmin-4 and oxytocin in hypothalamic programming of body weight and energy balance.
Zhang G, Bai H, Zhang H, Dean C, Wu Q, Li J, Guariglia S, Meng Q, Cai D
Neuron (2011) 693: 523-35. 105 043 IHC
Synaptotagmin IV: a multifunctional regulator of peptidergic nerve terminals.
Zhang Z, Bhalla A, Dean C, Chapman ER, Jackson MB
Nature neuroscience (2009) 122: 163-71. 105 043 WB, EM
Synaptotagmin-IV modulates synaptic function and long-term potentiation by regulating BDNF release.
Dean C, Liu H, Dunning FM, Chang PY, Jackson MB, Chapman ER
Nature neuroscience (2009) 126: 767-76. 105 043 WB, app.icc; ip
WB
Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.
Other products for this target