Synaptic Systems - Synaptotagmin3

Cat. No.: 105 133

Amount: 50 µg

Price: $375.00

Cat. No. 105 133	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 123 to 154 from human Synaptotagmin3 (UniProt Id: Q9BQG1)
Reactivity	Reacts with: rat (P40748), mouse (O35681), human (Q9BQG1). Other species not tested yet.
Specificity	K.O. validated PubMed: 30545844
Data sheet	105_133.pdf

References for Synaptotagmin3 - 105 133

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 105 133 WB, IHC; KO verified; tested species: mouse

Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 133 ICC

Synaptotagmins 3 and 7 mediate the majority of asynchronous release from synapses in the cerebellum and hippocampus.
Weingarten DJ, Shrestha A, Orlin DJ, Le Moing CL, Borchardt LA, Jackman SL
Cell reports (2024) 438: 114595. 105 133 IHC; KO verified; tested species: mouse

Cat. No.: 105 133

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

Up to now at least 17 synaptotagmins have been identified. Like other proteins of this family synaptotagmin3 is composed of a vesicular, a transmembrane and two C2 domains.
Synaptotagmin3 is expressed in brain, where it localizes to synaptic plasma membranes, and also in non-neuronal cells. In pancreatic islet β-cells it is involved in Ca²⁺ triggered insulin secretion, and in basophilic leukemia mast cells (RBL-cells) in transport events to the perinuclear endocytic recycling compartment.

1 ) Grimberg E et al., J. Cell. Sci. (2003)
2 ) Gao Z et al., J. Biol. Chem. (2000)
3 ) Butz S et al., J. Biol. Chem. (1999)
4 ) Li C et al., Nature (1995)
5 ) Hilbush BS et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

ICC

IHC

Other products for this target

Synaptotagmin3 antibody - 105 133 K.O.

References for Synaptotagmin3 - 105 133