Synaptotagmin3 antibody - 105 033

Synaptotagmin3 is enriched in synaptosomal membranes

Rabbit polyclonal purified antibody

Cat. No.: 105 033

Amount: 50 µg

Price: $375.00

Cat. No. 105 033	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 180 to 293 from human Synaptotagmin3 (UniProt Id: Q9BQG1)
Reactivity	Reacts with: human (Q9BQG1), rat (P40748), mouse (O35681). Other species not tested yet.
Data sheet	105_033.pdf

Cat. No.: 105 033

Amount: 50 µg

Price: $375.00

ICC

Background

Up to now at least 17 synaptotagmins have been identified. Like other proteins of this family synaptotagmin3 is composed of a vesicular, a transmembrane and two C2 domains.
Synaptotagmin3 is expressed in brain, where it localizes to synaptic plasma membranes, and also in non-neuronal cells. In pancreatic islet β-cells it is involved in Ca²⁺ triggered insulin secretion, and in basophilic leukemia mast cells (RBL-cells) in transport events to the perinuclear endocytic recycling compartment.

1 ) Grimberg E et al., J. Cell. Sci. (2003)
2 ) Gao Z et al., J. Biol. Chem. (2000)
3 ) Butz S et al., J. Biol. Chem. (1999)
4 ) Li C et al., Nature (1995)
5 ) Hilbush BS et al., Proc. Natl. Acad. Sci. U.S.A. (1994)

Protocols

ICC

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