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Synaptotagmin2 antibody luminal domain - 105 225

Synaptotagmin2 is an alternative Ca2+-sensor for neurotransmitter release
Guinea pig polyclonal purified antibody
Cat. No.: 105 225
Amount: 50 µg
Price: $460.00
Cat. No. 105 225 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 AP staining gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide corresponding to AA 1 to 11 from mouse Synaptotagmin2 (UniProt Id: P46097)
Reactivity Reacts with: mouse (P46097), rat (P29101).
Other species not tested yet.
Data sheet 105_225.pdf

References for Synaptotagmin2 - 105 225

Simulations of active zone structure and function at mammalian NMJs predict that loss of calcium channels alone is not sufficient to replicate LEMS effects.
Ginebaugh SP, Badawi Y, Laghaei R, Mersky G, Wallace CJ, Tarr TB, Kaufhold C, Reddel S, Meriney SD
Journal of neurophysiology (2023) : . 105 225 IHC; tested species: mouse
Cat. No.: 105 225
Amount: 50 µg
Price: $460.00
Simulations of active zone structure and function at mammalian NMJs predict that loss of calcium channels alone is not sufficient to replicate LEMS effects.
Ginebaugh SP, Badawi Y, Laghaei R, Mersky G, Wallace CJ, Tarr TB, Kaufhold C, Reddel S, Meriney SD
Journal of neurophysiology (2023) : . 105 225 IHC; tested species: mouse
Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca2+ sensor for fast secretion.