Synaptic Systems - Synaptotagmin2

Cat. No.: 105 223

Amount: 50 µg

Price: $460.00

Cat. No. 105 223	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 (see remarks) gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 11 from mouse Synaptotagmin2 (UniProt Id: P46097)
Reactivity	Reacts with: rat (P29101), mouse (P46097). Other species not tested yet.
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_223.pdf

References for Synaptotagmin2 - 105 223

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 105 223 IHC; tested species: mouse

The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus.
Wen L, Yang X, Wu Z, Fu S, Zhan Y, Chen Z, Bi D, Shen Y
Cell reports (2023) 424: 112349. 105 223 IHC; tested species: mouse

Chronic partial TrkB activation reduces seizures and mortality in a mouse model of Dravet syndrome.
Gu F, Parada I, Yang T, Longo FM, Prince DA
Proceedings of the National Academy of Sciences of the United States of America (2022) 1197: . 105 223 IHC; tested species: mouse

Myelination synchronizes cortical oscillations by consolidating parvalbumin-mediated phasic inhibition.
Dubey M, Pascual-Garcia M, Helmes K, Wever DD, Hamada MS, Kushner SA, Kole MHP
eLife (2022) 11: . 105 223 IHC; tested species: mouse

ROCK/PKA inhibition rescues hippocampal hyperexcitability and GABAergic neuron alterations in Oligophrenin-1 Knock-out mouse model of X-linked intellectual disability.
Busti I, Allegra M, Spalletti C, Panzi C, Restani L, Billuart P, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 105 223 IHC; tested species: mouse

Inositol polyphosphate multikinase deficiency leads to aberrant induction of synaptotagmin-2 in the forebrain.
Park J, Park SJ, Kim S
Molecular brain (2019) 121: 58. 105 223 IHC; tested species: mouse

Control of exocytosis by synaptotagmins and otoferlin in auditory hair cells.
Beurg M, Michalski N, Safieddine S, Bouleau Y, Schneggenburger R, Chapman ER, Petit C, Dulon D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13281-90. 105 223 IHC; tested species: rat

Cat. No.: 105 223

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca²⁺ sensor for fast secretion.

1 ) Pang ZP et al., J. Neurosci. (2006)
2 ) Pang ZP et al., EMBO J. (2006)
3 ) Lee BH et al., Mol. Cell (2004)
4 ) Lindmark IM et al., Biochim. Biophys. Acta (2002)
5 ) Kida Y et al., FEBS Lett. (2001)
6 ) Baram D et al., J. Exp. Med. (1999)
7 ) Geppert M et al., J. Biol. Chem. (1991)

Protocols

Standard protocol

ICC

IHC

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References for Synaptotagmin2 - 105 223