Synaptotagmin2 antibody luminal domain - 105 222

Synaptotagmin2 is an alternative Ca²⁺-sensor for neurotransmitter release

Rabbit polyclonal antiserum

Cat. No.: 105 222

Amount: 200 µl

Price: $355.00

Cat. No. 105 222	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 (see remarks) gallery IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1 to 11 from mouse Synaptotagmin2 (UniProt Id: P46097)
Reactivity	Reacts with: rat (P29101), mouse (P46097). Other species not tested yet.
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_222.pdf

Cat. No.: 105 222

Amount: 200 µl

Price: $355.00

ICC

Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca²⁺ sensor for fast secretion.

1 ) Pang ZP et al., J. Neurosci. (2006)
2 ) Pang ZP et al., EMBO J. (2006)
3 ) Lee BH et al., Mol. Cell (2004)
4 ) Lindmark IM et al., Biochim. Biophys. Acta (2002)
5 ) Kida Y et al., FEBS Lett. (2001)
6 ) Baram D et al., J. Exp. Med. (1999)
7 ) Geppert M et al., J. Biol. Chem. (1991)

Protocols

Standard protocol

ICC

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