Synaptic Systems - Synaptotagmin2

Cat. No.: 105 123

Amount: 50 µg

Price: $375.00

Cat. No. 105 123	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: yes IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 406 to 422 from rat Synaptotagmin2 (UniProt Id: P29101)
Reactivity	Reacts with: human (Q8N9I0), rat (P29101), mouse (P46097). Other species not tested yet.
Matching control protein/peptide	105-12P
Data sheet	105_123.pdf

References for Synaptotagmin2 - 105 123

SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 105 123 WB; tested species: mouse

Paroxysmal and cognitive phenotypes in Prrt2 mutant mice.
Robertson L, Featherby T, Howell S, Hughes J, Thomas P
Genes, brain, and behavior (2019) : e12566. 105 123 WB; tested species: mouse

Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models.
Elliott M, Favre-Guilmard C, Liu SM, Maignel J, Masuyer G, Beard M, Boone C, Carré D, Kalinichev M, Lezmi S, Mir I, et al.
Science advances (2019) 51: eaau7196. 105 123 WB; tested species: mouse

Proteomic analysis of wild-type and mutant huntingtin-associated proteins in mouse brains identifies unique interactions and involvement in protein synthesis.
Culver BP, Savas JN, Park SK, Choi JH, Zheng S, Zeitlin SO, Yates JR, Tanese N
The Journal of biological chemistry (2012) 28726: 21599-614. 105 123 WB

Novel application of human neurons derived from induced pluripotent stem cells for highly sensitive botulinum neurotoxin detection.
Whitemarsh RC, Strathman MJ, Chase LG, Stankewicz C, Tepp WH, Johnson EA, Pellett S
Toxicological sciences : an official journal of the Society of Toxicology (2012) 1262: 426-35. 105 123 WB

Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin.
Pellett S, Schwartz MP, Tepp WH, Josephson R, Scherf JM, Pier CL, Thomson JA, Murphy WL, Johnson EA
Scientific reports (2015) 5: 14566. 105 123 ICC

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 105 123 ICC; tested species: rat

Calcium is Reduced in Presynaptic Mitochondria of Motor Nerve Terminals during Neurotransmission in SMA Mice.
Lopez-Manzaneda M, Franco-Espin J, Tejero R, Cano R, Tabares L
Human molecular genetics (2021) : . 105 123 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 105 123 IHC; tested species: rat

Loss of β2-laminin alters calcium sensitivity and voltage-gated calcium channel maturation of neurotransmission at the neuromuscular junction.
Chand KK, Lee KM, Schenning MP, Lavidis NA, Noakes PG
The Journal of physiology (2015) 5931: 245-65. 105 123 IHC

Synaptotagmin IV determines the linear Ca2+ dependence of vesicle fusion at auditory ribbon synapses.
Johnson SL, Franz C, Kuhn S, Furness DN, Rüttiger L, Münkner S, Rivolta MN, Seward EP, Herschman HR, Engel J, Knipper M, et al.
Nature neuroscience (2010) 131: 45-52. 105 123 IHC

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 105 123 DNA-PAINT; tested species: rat

Cat. No.: 105 123

Amount: 50 µg

Price: $375.00

Paroxysmal and cognitive phenotypes in Prrt2 mutant mice.
Robertson L, Featherby T, Howell S, Hughes J, Thomas P
Genes, brain, and behavior (2019) : e12566. 105 123 WB; tested species: mouse

Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca²⁺ sensor for fast secretion.

1 ) Pang ZP et al., J. Neurosci. (2006)
2 ) Pang ZP et al., EMBO J. (2006)
3 ) Lee BH et al., Mol. Cell (2004)
4 ) Lindmark IM et al., Biochim. Biophys. Acta (2002)
5 ) Kida Y et al., FEBS Lett. (2001)
6 ) Baram D et al., J. Exp. Med. (1999)
7 ) Geppert M et al., J. Biol. Chem. (1991)

Protocols

Standard protocol

ICC

OTHER

Preabsorbtion

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Synaptotagmin2 antibody cytoplasmic domain - 105 123

References for Synaptotagmin2 - 105 123