Synaptotagmin2 peptide cytoplasmic domain - 105-12P

Synaptotagmin2 is an alternative Ca²⁺-sensor for neurotransmitter release

control peptide

Cat. No.: 105-12P

Amount: 100 µg

Price: $105.00

Cat. No. 105-12P	100 µg peptide, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized!
Applications
Immunogen	Synthetic peptide corresponding to AA 406 to 422 from rat Synaptotagmin2 (UniProt Id: P29101)
Recommended dilution	Optimal concentrations should be determined by the end-user.
Matching antibodies	105 123
Remarks	This control peptide consists of the synthetic peptide (aa 406-422 in rat synaptotagmin 2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
Data sheet	105-12p.pdf

Cat. No.: 105-12P

Amount: 100 µg

Price: $105.00

Background

Synaptotagmin2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin1.
Recently synaptotagmin2 has been shown to be an alternative Ca²⁺ sensor for fast secretion.

1 ) Pang ZP et al., J. Neurosci. (2006)
2 ) Pang ZP et al., EMBO J. (2006)
3 ) Lee BH et al., Mol. Cell (2004)
4 ) Lindmark IM et al., Biochim. Biophys. Acta (2002)
5 ) Kida Y et al., FEBS Lett. (2001)
6 ) Baram D et al., J. Exp. Med. (1999)
7 ) Geppert M et al., J. Biol. Chem. (1991)

Protocols

OTHER

Preadsorbtion

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