Synaptic Systems - Synaptotagmin11

Cat. No.: 270 003

Amount: 50 µg

Price: $380.00

Cat. No. 270 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: 1 : 500 IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 70 to 154 from mouse Synaptotagmin11 (UniProt Id: Q9R0N3)
Reactivity	Reacts with: rat (O08835), mouse (Q9R0N3). Other species not tested yet.
Specificity	K.D. validated PubMed: 28686317
Matching control protein/peptide	270-0P
Data sheet	270_003.pdf

References for Synaptotagmin11 - 270 003

Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles.
Trovò L, Kouvaros S, Schwenk J, Fernandez-Fernandez D, Fritzius T, Rem PD, Früh S, Gassmann M, Fakler B, Bischofberger J, Bettler B, et al.
EMBO reports (2024) : . 270 003 WB, ICC; tested species: mouse,human

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 270 003 WB; tested species: mouse

Synaptotagmin-11 inhibits cytokine secretion and phagocytosis in microglia.
Du C, Wang Y, Zhang F, Yan S, Guan Y, Gong X, Zhang T, Cui X, Wang X, Zhang CX
Glia (2017) 6510: 1656-1667. 270 003 WB; KD verified; tested species: mouse

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 270 003 ICC, WB

Injured astrocytes are repaired by Synaptotagmin XI-regulated lysosome exocytosis.
Sreetama SC, Takano T, Nedergaard M, Simon SM, Jaiswal JK
Cell death and differentiation (2016) 234: 596-607. 270 003 WB, ICC; tested species: mouse

Synaptotagmin-11 deficiency mediates schizophrenia-like behaviors in mice via dopamine over-transmission.
Chen Y, Gu Y, Wang B, Wei A, Dong N, Jiang Y, Liu X, Zhu L, Zhu F, Tan T, Jing Z, et al.
Nature communications (2024) 151: 10571. 270 003 IHC; tested species: mouse

Activity-dependent formation of a vesicular inhibitory amino acid transporter gradient in the superior olivary complex of NMRI mice.
Ebbers L, Weber M, Nothwang HG
BMC neuroscience (2017) 181: 75. 270 003 IHC; tested species: mouse

Cat. No.: 270 003

Amount: 50 µg

Price: $380.00

Background

Up to now at least 17 synaptotagmins have been identified. Synaptotagmin11, a close relative of synaptotagmin4, is mainly expressed in the brain. It is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
Synaptotagmin11 has been shown to localize mainly to excitatory and inhibitory presynaptic terminals.

1 ) Yeo H et al., Neuroscience (2012)
2 ) Mittelsteadt T et al., J. Comp. Neurol. (2009)
3 ) Inoue S et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. (2007)
4 ) Glass AS et al., J. Neural Transm. Suppl. (2004)
5 ) Huynh DP et al., Hum. Mol. Genet. (2003)

Protocols

ICC

OTHER

Preabsorbtion

Other products for this target

Synaptotagmin11 antibody - 270 003 K.D.

References for Synaptotagmin11 - 270 003