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Home > Products > 105 011C3

Synaptotagmin1 antibody cytoplasmic tail - 105 011C3 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 011C3
Amount: 100 µg
Price: $470.00
Cat. No. 105 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 500      gallery  
IHC-P: 1 : 500 gallery  
Label Sulfo-Cyanine 3
Clone 41.1
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: human (P21579), rat (P21707), mouse (P46096), mammals, zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 30038018
Data sheet 105_011c3.pdf

References for Synaptotagmin1 - 105 011C3

Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 011C3 ICC; KO verified; tested species: rat
Ca2+ sensor synaptotagmin-1 mediates exocytosis in mammalian photoreceptors.
Grassmeyer JJ, Cahill AL, Hays CL, Barta C, Quadros RM, Gurumurthy CB, Thoreson WB
eLife (2019) 8: . 105 011C3 IHC; KO verified; tested species: mouse
Cat. No.: 105 011C3
Amount: 100 µg
Price: $470.00
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 011C3 ICC; KO verified; tested species: rat
Ca2+ sensor synaptotagmin-1 mediates exocytosis in mammalian photoreceptors.
Grassmeyer JJ, Cahill AL, Hays CL, Barta C, Quadros RM, Gurumurthy CB, Thoreson WB
eLife (2019) 8: . 105 011C3 IHC; KO verified; tested species: mouse
ICC
IHC
IHC
IHC-P
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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