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Home > Products > 101 011C5

Synaptophysin1 antibody - 101 011C5 K.O.

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
Mouse monoclonal purified IgG
Cat. No.: 101 011C5
Amount: 50 µg
Price: $475.00
Cat. No. 101 011C5 50 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 5.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.
Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label Sulfo-Cyanine 5
Clone 7.2
Subtype IgG1 (λ light chain)
Immunogen Full-length recombinant rat synaptophysin (UniProt Id: P07825)
Epitop AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity K.O. validated
Remarks

Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
Data sheet 101_011c5.pdf

References for Synaptophysin1 - 101 011C5

Balance of Go1α and Go2α expression regulates motor function via the striatal dopaminergic system.
Baron J, Bilbao A, Hörtnagl H, Birnbaumer L, Leixner S, Spanagel R, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2018) : . 101 011C5 WB; tested species: mouse
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 101 011C5 ICC; tested species: mouse
Time-dependent reversal of synaptic plasticity induced by physiological concentrations of oligomeric Aβ42: an early index of Alzheimer's disease.
Koppensteiner P, Trinchese F, Fà M, Puzzo D, Gulisano W, Yan S, Poussin A, Liu S, Orozco I, Dale E, Teich AF, et al.
Scientific reports (2016) 6: 32553. 101 011C5 ICC
Structural plasticity of perisynaptic astrocyte processes involves ezrin and metabotropic glutamate receptors.
Lavialle M, Aumann G, Anlauf E, Pröls F, Arpin M, Derouiche A
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: 12915-9. 101 011C5 ICC; tested species: rat
Cat. No.: 101 011C5
Amount: 50 µg
Price: $475.00
Balance of Go1α and Go2α expression regulates motor function via the striatal dopaminergic system.
Baron J, Bilbao A, Hörtnagl H, Birnbaumer L, Leixner S, Spanagel R, Ahnert-Hilger G, Brunk I
Journal of neurochemistry (2018) : . 101 011C5 WB; tested species: mouse
Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.
Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ, et al.
The EMBO journal (2020) : e103358. 101 011C5 ICC; tested species: mouse
Time-dependent reversal of synaptic plasticity induced by physiological concentrations of oligomeric Aβ42: an early index of Alzheimer's disease.
Koppensteiner P, Trinchese F, Fà M, Puzzo D, Gulisano W, Yan S, Poussin A, Liu S, Orozco I, Dale E, Teich AF, et al.
Scientific reports (2016) 6: 32553. 101 011C5 ICC
Structural plasticity of perisynaptic astrocyte processes involves ezrin and metabotropic glutamate receptors.
Lavialle M, Aumann G, Anlauf E, Pröls F, Arpin M, Derouiche A
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: 12915-9. 101 011C5 ICC; tested species: rat
ICC
IHC
Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.
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