Synaptogyrin2 antibody - 103 203

Synaptogyrin2 is an ubiquitiously expressed Synaptogyrin isoform

Rabbit polyclonal purified antibody

Cat. No.: 103 203

Amount: 50 µg

Price: $375.00

Cat. No. 103 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 2000 AP-staining gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 211 to 224 from rat Synaptogyrin2 (UniProt Id: O54980)
Reactivity	Reacts with: rat (O54980), mouse (O55101). Other species not tested yet.
Matching control protein/peptide	103-02P
Data sheet	103_203.pdf

Cat. No.: 103 203

Amount: 50 µg

Price: $375.00

Background

Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.

1 ) Belfort GM et al., J. Biol. Chem. (2005)
2 ) Kupriyanova TA et al., J. Biol. Chem. (2000)
3 ) Janz R et al., J. Biol. Chem. (1998)

Protocols

OTHER

Preabsorbtion

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