Synaptic Systems - Synaptogyrin1

Cat. No.: 103 003

Amount: 50 µg

Price: $455.00

Cat. No. 103 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 220 to 234 from rat Synaptogyrin1 (UniProt Id: Q62876)
Reactivity	Reacts with: rat (Q62876), mouse (O55100). Other species not tested yet.
Matching control protein/peptide	103-0P
Data sheet	103_003.pdf

References for Synaptogyrin1 - 103 003

Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 103 003 WB; tested species: rat

Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 103 003 WB; tested species: mouse,rat

Cat. No.: 103 003

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.

1 ) Janz R et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Janz R et al., J. Biol. Chem. (1998)
4 ) Südhof TC et al., Nature (1995)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Synaptogyrin1 antibody - 103 003

References for Synaptogyrin1 - 103 003