Synaptic Systems

Cat. No.: 140 023

Amount: 50 µg

Price: $375.00

Cat. No. 140 023	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 955 to 1056 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity	Reacts with: rat (Q9JIR4), mouse (Q99NE5). Other species not tested yet.
Matching control protein/peptide	140-02P
Remarks	WB: This antibody is recommended.
Data sheet	140_023.pdf

References for RIM1 - 140 023

cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
Wang XT, Zhou L, Dong BB, Xu FX, Wang DJ, Shen EW, Cai XY, Wang Y, Wang N, Ji SJ, Chen W, et al.
eLife (2023) 12: . 140 023 WB; tested species: mouse

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 140 023 WB; tested species: mouse

Ethanol Mediated Inhibition of Synaptic Vesicle Recycling at Amygdala Glutamate Synapses Is Dependent upon Munc13-2.
Gioia DA, Alexander N, McCool BA
Frontiers in neuroscience (2017) 11: 424. 140 023 WB; tested species: mouse

Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 023 WB, IHC; tested species: mouse

Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 140 023 IHC; tested species: rat

Cat. No.: 140 023

Amount: 50 µg

Price: $375.00

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.

1 ) Wang Y et al., Genomics (2003)
2 ) Castillo PE et al., Nature (2002)
3 ) Schoch S et al., Nature (2002)
4 ) Wang Y et al., J. Biol. Chem. (2000)
5 ) Wang Y et al., Nature (1997)

Protocols

OTHER

Preabsorbtion

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RIM1 antibody - 140 023

References for RIM1 - 140 023