RIM1 antibody PDZ domain - 140 005

RIMs are Rab3 effector proteins of the cytomatrix at the active zone

Guinea pig polyclonal purified antibody

Cat. No.: 140 005

Amount: 50 µg

Price: $460.00

Cat. No. 140 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 596 to 705 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity	Reacts with: rat (Q9JIR4), mouse (Q99NE5). Other species not tested yet.
Specificity	Specific for RIM 1 with weak cross reactivity to RIM 2.
Remarks	IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
Data sheet	140_005.pdf

References for RIM1 - 140 005

Correction of amygdalar dysfunction in a rat model of fragile X syndrome.
Fernandes G, Mishra PK, Nawaz MS, Donlin-Asp PG, Rahman MM, Hazra A, Kedia S, Kayenaat A, Songara D, Wyllie DJA, Schuman EM, et al.
Cell reports (2021) 372: 109805. 140 005 IHC; tested species: rat

Cat. No.: 140 005

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.

1 ) Wang Y et al., Genomics (2003)
2 ) Castillo PE et al., Nature (2002)
3 ) Schoch S et al., Nature (2002)
4 ) Wang Y et al., J. Biol. Chem. (2000)
5 ) Wang Y et al., Nature (1997)

Protocols

ICC

IHC

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