Cat. No.: 140 005
Amount: 50 µg
Price:
$460.00
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| Cat. No. 140 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to AA 596 to 705 from rat Rim1 (UniProt Id: Q9JIR4) |
| Reactivity |
Reacts with: rat (Q9JIR4), mouse (Q99NE5). Other species not tested yet. |
| Specificity | Specific for RIM 1 with weak cross reactivity to RIM 2. |
| Remarks |
IHC: This antibody requires antigen retrieval with pepsin according to: |
| Data sheet | 140_005.pdf |
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RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.