Synaptic Systems

Cat. No.: 192 104

Amount: 100 µl

Price: $365.00

Cat. No. 192 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 10000 (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 95 to 207 from rat Ribeye (UniProt Id: Q9EQH5-2)
Reactivity	Reacts with: rat (Q9EQH5-2), mouse (P56546-2). Other species not tested yet.
Specificity	This antibody recognizes only ribeye and not CtBP 2.
Remarks	IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 or Klotz et al. 2019 is recommended.
Data sheet	192_104.pdf

References for Ribeye - 192 104

The Role of Sphingosine-1-Phosphate Receptor 2 in Mouse Retina Light Responses.
Shrestha AP, Stiles M, Grambergs RC, Boff JM, Madireddy S, Mondal K, Rajmanna R, Porter H, Sherry DM, Proia RL, Vaithianathan T, et al.
Biomolecules (2023) 1312: . 192 104 IHC; tested species: mouse

Hyperacusis in the Adult Fmr1-KO Mouse Model of Fragile X Syndrome: The Therapeutic Relevance of Cochlear Alterations and BKCa Channels.
Ferraguto C, Bouleau Y, Peineau T, Dulon D, Pietropaolo S
International journal of molecular sciences (2023) 2414: . 192 104 IHC; tested species: mouse

Glutamate transporters EAAT2 and EAAT5 differentially shape synaptic transmission from rod bipolar cell terminals.
Tang FS, Yuan HL, Liu JB, Zhang G, Chen SY, Ke JB
eNeuro (2022) : . 192 104 IHC; tested species: mouse

The SNARE protein SNAP-25 is required for normal exocytosis at auditory hair cell ribbon synapses.
Calvet C, Peineau T, Benamer N, Cornille M, Lelli A, Plion B, Lahlou G, Fanchette J, Nouaille S, Boutet de Monvel J, Estivalet A, et al.
iScience (2022) 2512: 105628. 192 104 IHC; tested species: mouse

Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 192 104 IHC; tested species: mouse

Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes.
Ryl M, Urbasik A, Gierke K, Babai N, Joachimsthaler A, Feigenspan A, Frischknecht R, Stallwitz N, Fejtová A, Kremers J, von Wittgenstein J, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2021) 355: e21520. 192 104 IHC; tested species: mouse

Localization of group II and III metabotropic glutamate receptors at pre- and postsynaptic sites of inner hair cell ribbon synapses.
Klotz L, Wendler O, Frischknecht R, Shigemoto R, Schulze H, Enz R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201901543R. 192 104 IHC; tested species: mouse

Cat. No.: 192 104

Amount: 100 µl

Price: $365.00

IHC

IHC-P

Background

The photoreceptor ribbon synapse is a unique type of synapse specialized for the tonic release of neurotransmitter in the dark. Ribeye is a self-aggregating protein and is one of the major scaffolding components of the ribbon on which the neurotransmitter containing vesicles are tethered. The protein consists of a unique A-domain and a B-domain. With the exception of the first 20 amino acids the B-domain is identical to the transcriptional corepressor CtBP 2. Both proteins originate from the same gene.

1 ) Verger A et al., Mol. Cell. Biol. (2006)
2 ) Brandt A et al., J. Neurosci. (2005)
3 ) tom Dieck S et al., J. Cell Biol. (2005)
4 ) Schmitz F et al., Neuron (2000)
5 ) Furusawa T et al., Mol. Cell. Biol. (1999)

Protocols

IHC

IHC-P

Other products for this target

Ribeye antibody A-domain - 192 104

References for Ribeye - 192 104