Synaptic Systems

Cat. No.: 409 011

Amount: 100 µg

Price: $425.00

Cat. No. 409 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	337B1
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 219 from sea anemone mScarlet
Specificity	Recognizes mRFP, mCherry, mOrgange2, tdTomato, mScarlet.
Data sheet	409_011.pdf

References for RFP - 409 011

Isolation and tracing of matrix-producing notochordal and chondrocyte cells using ACAN-2A-mScarlet reporter human iPSC lines.
Tong X, Poramba-Liyanage DW, van Hoolwerff M, Riemers FM, Montilla-Rojo J, Warin J, Salvatori D, Camus A, Meulenbelt I, Ramos YFM, Geijsen N, et al.
Science advances (2024) 1043: eadp3170. 409 011 ICC; tested species: human

Presynaptic Rac1 in the hippocampus selectively regulates working memory.
Kim J, Bustamante E, Sotonyi P, Maxwell N, Parameswaran P, Kent JK, Wetsel WC, Soderblom EJ, Rácz B, Soderling SH
eLife (2024) 13: . 409 011 ICC; tested species: mouse

Cat. No.: 409 011

Amount: 100 µg

Price: $425.00

ICC

IHC

Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D et al., Genesis (2010)
2 ) Shaner NC et al., Nat. Biotechnol. (2004)
3 ) Labas YA et al., Proc. Natl. Acad. Sci. U.S.A. (2002)
4 ) Fradkov AF et al., FEBS Lett. (2000)

Protocols

ICC

IHC

Other products for this target

RFP antibody - 409 011

References for RFP - 409 011