RFP antibody - 390 011

RFP is a red fluorescent protein tag

Mouse monoclonal purified IgG

Cat. No.: 390 011

Amount: 100 µg

Price: $415.00

Cat. No. 390 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet
Clone	115D6
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)
Specificity	Recognizes mRFP, mCherry, mOrgange2, and Scarlet. Does not detect dsRed and tdTomato.
Data sheet	390_011.pdf

Cat. No.: 390 011

Amount: 100 µg

Price: $415.00

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Background

Red fluorescent protein RFP and its derivates have become universal tools in cell biology. Most RFPs derive from a protein isolated from Discosoma sp. They are used as fluorescent tags to investigate expression levels, patterns and protein localization.

1 ) Fink D, Genesis (New York, N.Y. : 2000) (2010) 4812: 723-9.
2 ) Shaner NC, Nature biotechnology (2004) 2212: 1567-72.
3 ) Labas YA, Proceedings of the National Academy of Sciences of the United States of America (2002) 997: 4256-61.
4 ) Fradkov AF, FEBS letters (2000) 4793: 127-30.

Protocols

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