Synaptic Systems - Rabphilin3a

Cat. No.: 118 002

Amount: 200 µl

Price: $360.00

Cat. No. 118 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: not tested yet IHC: yes IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 671 to 684 from rat Rabphilin3a (UniProt Id: P47709)
Reactivity	Reacts with: human (Q9Y2J0), rat (P47709), mouse (P47708), cow. Other species not tested yet.
Matching control protein/peptide	118-0P
Data sheet	118_002.pdf

References for Rabphilin3a - 118 002

SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.
Chamberlain LH, Burgoyne RD, Gould GW
Proceedings of the National Academy of Sciences of the United States of America (2001) 9810: 5619-24. 118 002 WB; tested species: rat

Novel localization of Rab3D in rat intestinal goblet cells and Brunner's gland acinar cells suggests a role in early Golgi trafficking.
Valentijn JA, van Weeren L, Ultee A, Koster AJ
American journal of physiology. Gastrointestinal and liver physiology (2007) 2931: G165-77. 118 002 IHC; tested species: rat

Cat. No.: 118 002

Amount: 200 µl

Price: $360.00

Background

Rabphilin 3a is a putative effector protein for the low molecular weight GTP-binding protein rab 3. Rab 3 occurs in four isoforms (Rab 3a, b, c and d), all of which probably bind to rabphilin 3a when in the GTP-bound form.
Rabphilin 3a contains an N-terminal Zn²⁺-finger sequence that is essential for binding rab 3, and two C-terminal C2 - domains that may bind Ca²⁺. It does not have a transmembrane region.
Rabphilin 3a is primarily expressed in neurons where it is localized to synaptic vesicles. It is probably recruited to synaptic vesicles by rab 3a and 3c. The structure of rabphilin 3a and its interaction with rab 3 suggests that it may be a Ca²⁺ sensor on synaptic vesicles that is recruited to synaptic vesicles as a function of GTP by rab 3.

1 ) Schlüter OM et al., J. Neurosci. (1999)
2 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
3 ) Stahl B et al., EMBO J. (1996)
4 ) Li C et al., Neuron (1994)
5 ) Shirataki H et al., Mol. Cell. Biol. (1993)

Protocols

IHC

OTHER

Preabsorbtion

Other products for this target

Rabphilin3a antibody - 118 002

References for Rabphilin3a - 118 002