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Home > Products > 130 011

Rab-GDI1 antibody - 130 011

The cytoplasmatic protein Rab-GDI binds to GDP-Rabs
Mouse monoclonal purified IgG
Cat. No.: 130 011
Amount: 100 µg
Price: $415.00
Cat. No. 130 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not tested yet
IHC: not recommended
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 81.2
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 447 from bovine Rab-GDI1 (UniProt Id: P21856)
Reactivity Reacts with: human (P31150), rat (P50398), mouse (P50396), hamster, cow.
Other species not tested yet.
Specificity Recognizes Rab-GDI-α and -β.
Remarks

IP: Immunoprecipitates Rab-GDI complexes.
Data sheet 130_011.pdf

References for Rab-GDI1 - 130 011

Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 130 011 WB; tested species: rat
Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins.
Afuwape OAT, Chanaday NL, Kasap M, Monteggia LM, Kavalali ET
The Journal of physiology (2024) : . 130 011 WB; tested species: mouse
Interneuronal exchange and functional integration of synaptobrevin via extracellular vesicles.
Vilcaes AA, Chanaday NL, Kavalali ET
Neuron (2021) 1096: 971-983.e5. 130 011 WB; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 130 011 WB; tested species: mouse
Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140.
Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, Schaub T, et al.
Neuron (2018) : . 130 011 WB; tested species: mouse
Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Crawford DC, Ramirez DM, Trauterman B, Monteggia LM, Kavalali ET
Nature communications (2017) 8: 14436. 130 011 WB
ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion.
Huang YA, Zhou B, Wernig M, Südhof TC
Cell (2017) 1683: 427-441.e21. 130 011 WB; tested species: mouse
Loss of Doc2-Dependent Spontaneous Neurotransmission Augments Glutamatergic Synaptic Strength.
Ramirez DMO, Crawford DC, Chanaday NL, Trauterman B, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3726: 6224-6230. 130 011 WB; tested species: rat
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 130 011 WB; tested species: mouse
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 130 011 WB
Analysis of conditional heterozygous STXBP1 mutations in human neurons.
Patzke C, Han Y, Covy J, Yi F, Maxeiner S, Wernig M, Südhof TC
The Journal of clinical investigation (2015) 1259: 3560-71. 130 011 WB
Alpha-Synuclein Expression Restricts RNA Viral Infections in the Brain.
Beatman EL, Massey A, Shives KD, Burrack KS, Chamanian M, Morrison TE, Beckham JD
Journal of virology (2015) 906: 2767-82. 130 011 WB; tested species: mouse
α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation.
Burré J, Sharma M, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2014) 11140: E4274-83. 130 011 WB
Ear2 deletion causes early memory and learning deficits in APP/PS1 mice.
Kummer MP, Hammerschmidt T, Martinez A, Terwel D, Eichele G, Witten A, Figura S, Stoll M, Schwartz S, Pape HC, Schultze JL, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3426: 8845-54. 130 011 WB; tested species: mouse
α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity.
Chen RH, Wislet-Gendebien S, Samuel F, Visanji NP, Zhang G, Marsilio D, Langman T, Fraser PE, Tandon A
The Journal of biological chemistry (2013) 28811: 7438-49. 130 011 WB
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 130 011 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 130 011 WB; tested species: mouse
Syntaxin-1 N-peptide and Habc-domain perform distinct essential functions in synaptic vesicle fusion.
Zhou P, Pang ZP, Yang X, Zhang Y, Rosenmund C, Bacaj T, Südhof TC
The EMBO journal (2013) 321: 159-71. 130 011 WB; tested species: mouse
RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction.
Kaeser PS, Deng L, Wang Y, Dulubova I, Liu X, Rizo J, Südhof TC
Cell (2011) 1442: 282-95. 130 011 WB; tested species: mouse
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 130 011 WB; tested species: mouse
Calmodulin controls synaptic strength via presynaptic activation of calmodulin kinase II.
Pang ZP, Cao P, Xu W, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3011: 4132-42. 130 011 WB; tested species: mouse
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Südhof TC
Science (New York, N.Y.) (2010) 3295999: 1663-7. 130 011 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 130 011 WB, IP
SynCAMs organize synapses through heterophilic adhesion.
Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
The Journal of neuroscience : the official journal of the Society for Neuroscience (2007) 2746: 12516-30. 130 011 WB
A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice.
Tabuchi K, Blundell J, Etherton MR, Hammer RE, Liu X, Powell CM, Südhof TC
Science (New York, N.Y.) (2007) 3185847: 71-6. 130 011 WB; tested species: mouse
A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 130 011 WB
Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term depression.
Huang CC, You JL, Wu MY, Hsu KS
The Journal of biological chemistry (2004) 27913: 12286-92. 130 011 WB
Binding of Rab3A to synaptic vesicles.
Chou JH, Jahn R
The Journal of biological chemistry (2000) 27513: 9433-40. 130 011 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 130 011 WB, IP
Proteomics of photoreceptor outer segments identifies a subset of SNARE and Rab proteins implicated in membrane vesicle trafficking and fusion.
Kwok MC, Holopainen JM, Molday LL, Foster LJ, Molday RS
Molecular & cellular proteomics : MCP (2008) 76: 1053-66. 130 011 IHC
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 130 011
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 130 011
Rabphilin knock-out mice reveal that rabphilin is not required for rab3 function in regulating neurotransmitter release.
Schlüter OM, Schnell E, Verhage M, Tzonopoulos T, Nicoll RA, Janz R, Malenka RC, Geppert M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (1999) 1914: 5834-46. 130 011
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 130 011
Cat. No.: 130 011
Amount: 100 µg
Price: $415.00
Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 130 011 WB; tested species: rat
Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins.
Afuwape OAT, Chanaday NL, Kasap M, Monteggia LM, Kavalali ET
The Journal of physiology (2024) : . 130 011 WB; tested species: mouse
Interneuronal exchange and functional integration of synaptobrevin via extracellular vesicles.
Vilcaes AA, Chanaday NL, Kavalali ET
Neuron (2021) 1096: 971-983.e5. 130 011 WB; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 130 011 WB; tested species: mouse
Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140.
Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, Schaub T, et al.
Neuron (2018) : . 130 011 WB; tested species: mouse
Selective molecular impairment of spontaneous neurotransmission modulates synaptic efficacy.
Crawford DC, Ramirez DM, Trauterman B, Monteggia LM, Kavalali ET
Nature communications (2017) 8: 14436. 130 011 WB
ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion.
Huang YA, Zhou B, Wernig M, Südhof TC
Cell (2017) 1683: 427-441.e21. 130 011 WB; tested species: mouse
Loss of Doc2-Dependent Spontaneous Neurotransmission Augments Glutamatergic Synaptic Strength.
Ramirez DMO, Crawford DC, Chanaday NL, Trauterman B, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3726: 6224-6230. 130 011 WB; tested species: rat
Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation.
Patzke C, Acuna C, Giam LR, Wernig M, Südhof TC
The Journal of experimental medicine (2016) 2134: 499-515. 130 011 WB; tested species: mouse
The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 130 011 WB
Analysis of conditional heterozygous STXBP1 mutations in human neurons.
Patzke C, Han Y, Covy J, Yi F, Maxeiner S, Wernig M, Südhof TC
The Journal of clinical investigation (2015) 1259: 3560-71. 130 011 WB
Alpha-Synuclein Expression Restricts RNA Viral Infections in the Brain.
Beatman EL, Massey A, Shives KD, Burrack KS, Chamanian M, Morrison TE, Beckham JD
Journal of virology (2015) 906: 2767-82. 130 011 WB; tested species: mouse
α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation.
Burré J, Sharma M, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2014) 11140: E4274-83. 130 011 WB
Ear2 deletion causes early memory and learning deficits in APP/PS1 mice.
Kummer MP, Hammerschmidt T, Martinez A, Terwel D, Eichele G, Witten A, Figura S, Stoll M, Schwartz S, Pape HC, Schultze JL, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3426: 8845-54. 130 011 WB; tested species: mouse
α-Synuclein membrane association is regulated by the Rab3a recycling machinery and presynaptic activity.
Chen RH, Wislet-Gendebien S, Samuel F, Visanji NP, Zhang G, Marsilio D, Langman T, Fraser PE, Tandon A
The Journal of biological chemistry (2013) 28811: 7438-49. 130 011 WB
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 130 011 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 130 011 WB; tested species: mouse
Syntaxin-1 N-peptide and Habc-domain perform distinct essential functions in synaptic vesicle fusion.
Zhou P, Pang ZP, Yang X, Zhang Y, Rosenmund C, Bacaj T, Südhof TC
The EMBO journal (2013) 321: 159-71. 130 011 WB; tested species: mouse
RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction.
Kaeser PS, Deng L, Wang Y, Dulubova I, Liu X, Rizo J, Südhof TC
Cell (2011) 1442: 282-95. 130 011 WB; tested species: mouse
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 130 011 WB; tested species: mouse
Calmodulin controls synaptic strength via presynaptic activation of calmodulin kinase II.
Pang ZP, Cao P, Xu W, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3011: 4132-42. 130 011 WB; tested species: mouse
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Südhof TC
Science (New York, N.Y.) (2010) 3295999: 1663-7. 130 011 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 130 011 WB, IP
SynCAMs organize synapses through heterophilic adhesion.
Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T
The Journal of neuroscience : the official journal of the Society for Neuroscience (2007) 2746: 12516-30. 130 011 WB
A neuroligin-3 mutation implicated in autism increases inhibitory synaptic transmission in mice.
Tabuchi K, Blundell J, Etherton MR, Hammer RE, Liu X, Powell CM, Südhof TC
Science (New York, N.Y.) (2007) 3185847: 71-6. 130 011 WB; tested species: mouse
A dual function for Munc-18 in exocytosis of PC12 cells.
Schütz D, Zilly F, Lang T, Jahn R, Bruns D
The European journal of neuroscience (2005) 219: 2419-32. 130 011 WB
Rap1-induced p38 mitogen-activated protein kinase activation facilitates AMPA receptor trafficking via the GDI.Rab5 complex. Potential role in (S)-3,5-dihydroxyphenylglycene-induced long term depression.
Huang CC, You JL, Wu MY, Hsu KS
The Journal of biological chemistry (2004) 27913: 12286-92. 130 011 WB
Binding of Rab3A to synaptic vesicles.
Chou JH, Jahn R
The Journal of biological chemistry (2000) 27513: 9433-40. 130 011 WB
Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 130 011 WB, IP
Proteomics of photoreceptor outer segments identifies a subset of SNARE and Rab proteins implicated in membrane vesicle trafficking and fusion.
Kwok MC, Holopainen JM, Molday LL, Foster LJ, Molday RS
Molecular & cellular proteomics : MCP (2008) 76: 1053-66. 130 011 IHC
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 130 011
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 130 011
Rabphilin knock-out mice reveal that rabphilin is not required for rab3 function in regulating neurotransmitter release.
Schlüter OM, Schnell E, Verhage M, Tzonopoulos T, Nicoll RA, Janz R, Malenka RC, Geppert M, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (1999) 1914: 5834-46. 130 011
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 130 011
WB
Background

Rab-GDI forms complexes exclusively with the GDP-form of rab proteins. Complex formation shields the geranylgeranyl moieties of rabs and leads to their dissociation from the membrane.
There are several isoforms of rab-GDI which nondiscriminately interact with all rab proteins including rab 3 and rab 5 but not with other small GTPases such as members of the rho or ras families. GDI-α is predominantly expressed in brain whereas other isoforms are more generally distributed.