Cat. No.: 108 111
Amount: 100 µg
Price:
$415.00
Cat. No. 108 111 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 100 up to 1 : 1000 (see remarks) gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 50 up to 1 : 500 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet |
Clone | 621.4 |
Subtype | IgG2a (κ light chain) |
Immunogen | Recombinant protein corresponding to AA 1 to 215 from rat Rab5 (UniProt Id: O88565) |
Reactivity |
Reacts with: human (Q9UJ41), rat (M0RC99), mouse (Q9JM13). Other species not tested yet. |
Specificity | Specific for rab 5 (probably only rab 5a). No cross-reactivity to other rab proteins. |
Remarks |
This antibody was used very successfully for immunoisolation of early endosomes and for the differentiation of early endosomes from related trafficking organelles in neurons and nonneuronal cells. |
Data sheet | 108_111.pdf |