Cat. No.: 107 111BT
Amount: 100 µg
Price:
$465.00
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| Cat. No. 107 111BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: yes ICC: 1 : 1000 up to 1 : 2000 IHC: not recommended IHC-P: not tested yet ELISA: |
| Label | biotin |
| Clone | 42.2 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to AA 1 to 220 from rat Rab3a (UniProt Id: P63012) |
| Epitop |
AA 191 to 220 from rat Rab3a (UniProt Id: P63012) |
| Reactivity |
Reacts with: human (P20336), rat (P63012), mouse (P63011), mammals. Other species not tested yet. |
| Specificity | K.O. validated |
| Data sheet | 107_111bt.pdf |
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Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca2+ regulated excocytosis and converts it into an constitutive Ca2+ independent exocytosis mechanism.