Synaptic Systems

Cat. No.: 168 103

Amount: 50 µg

Price: $375.00

Cat. No. 168 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 400 gallery IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 218 from human Rab27b (UniProt Id: O00194)
Reactivity	Reacts with: rat (Q99P74), mouse (Q99P58), human (O00194). Other species not tested yet.
Specificity	Specific for rab 27B with weak cross reaction to rab 27A. K.O. validated PubMed: 26845357
Matching control protein/peptide	168-1P
Data sheet	168_103.pdf

References for Rab27B - 168 103

zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1.
Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ
JACC. Basic to translational science (2023) 85: 518-542. 168 103 WB; tested species: mouse

Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 103 WB; KD verified; tested species: human

Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 103 WB; KO verified

Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC

EPI64B acts as a GTPase-activating protein for Rab27B in pancreatic acinar cells.
Hou Y, Chen X, Tolmachova T, Ernst SA, Williams JA
The Journal of biological chemistry (2013) 28827: 19548-57. 168 103 WB

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC

Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes.
Shen YT, Gu Y, Su WF, Zhong JF, Jin ZH, Gu XS, Chen G
Neuroscience bulletin (2016) 324: 331-40. 168 103 ICC

Cat. No.: 168 103

Amount: 50 µg

Price: $375.00

ICC

IHC

IHC-P

Background

Rab 27 proteins are members of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes. Two Rab 27 isoforms, Rab 27A and 27B, have been described so far.
Mutations in the Rab 27A gene have been shown to be responsible for the Griscelli syndrome characterized by pigment dilution of the hair and an uncontrolled T-lymphocyte and macrophage activation. This disorder is probably due to the dysfunction of melanosomes in melanocytes and lytic granules in CTLs. Additionally Rab 27A is located on mature insulin granules of pancreatic β-cells and is expressed in the pigment epithelium and choriocapillaris of the retina.
In patients who suffer from Griscelli syndrome because of missense mutations in the Rab 27A gene, Rab 27B is upregulated and partially compensates for Rab 27A dysfunction. Rab 27B also regulates amylase secretion in parotid acinar cells.
Recently it has been shown that Rab 27 is also involved in synaptic transmission in C. elegans.

1 ) Mahoney TR et al., Mol. Biol. Cell (2006)
2 ) Jordens I et al., Pigment Cell Res. (2006)
3 ) Tsuboi T et al., J. Cell. Sci. (2006)
4 ) Aslan D et al., Pediatr Hematol Oncol ()
5 ) Izumi T et al., Meth. Enzymol. (2005)
6 ) Kasai K et al., J. Clin. Invest. (2005)
7 ) Aizawa T et al., J. Clin. Invest. (2005)
8 ) Chen X et al., Biochem. Biophys. Res. Commun. (2004)
9 ) Westbroek W et al., Pigment Cell Res. (2004)
10 ) Imai A et al., J. Cell. Sci. (2004)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Rab27B antibody - 168 103 K.O.

References for Rab27B - 168 103