Synaptic Systems

Cat. No.: 269 011

Amount: 100 µg

Price: $415.00

Cat. No. 269 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	163E12
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 256 from human Rab26 (UniProt Id: Q9ULW5)
Reactivity	Reacts with: human (Q9ULW5), rat (P51156), mouse (Q504M8). Other species not tested yet.
Data sheet	269_011.pdf

References for Rab26 - 269 011

The GTPase Rab26 links synaptic vesicles to the autophagy pathway.
Binotti B, Pavlos NJ, Riedel D, Wenzel D, Vorbrüggen G, Schalk AM, Kühnel K, Boyken J, Erck C, Martens H, Chua JJ, et al.
eLife (2015) 4: e05597. 269 011 WB, IP, ICC

A Super-Resolved View of the Alzheimer's Disease-Related Amyloidogenic Pathway in Hippocampal Neurons.
Yu Y, Gao Y, Winblad B, Tjernberg LO, Schedin-Weiss S
Journal of Alzheimer's disease : JAD (2021) 832: 833-852. 269 011 ICC; tested species: mouse

PLEKHG5 regulates autophagy, survival and MGMT expression in U251-MG glioblastoma cells.
Witte KE, Slotta C, Lütkemeyer M, Kitke A, Coras R, Simon M, Kaltschmidt C, Kaltschmidt B
Scientific reports (2020) 101: 21858. 269 011 ICC; tested species: human

Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 269 011 ICC

Cat. No.: 269 011

Amount: 100 µg

Price: $415.00

ICC

Background

Rab 26 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. These proteins are involved in intracellular fusion reactions of vesicles or organelles with their target membranes.
Rab 26 is involved in the transport from Golgi to secretory granules.

1 ) Li C et al., J. Biol. Chem. (2012)
2 ) Tian X et al., Mol. Cell. Biol. (2010)
3 ) Wagner AC et al., Biochem. Biophys. Res. Commun. (1995)

Protocols

Standard protocol

ICC

Rab26 antibody - 269 011

References for Rab26 - 269 011