Tailor-made Antibodies
and Tools for Life Science

PSD95 antibody PDZ domain - 124 011 K.O.

PSD 95 is a component of postsynaptic densities in central synapses
Mouse monoclonal purified IgG
Cat. No.: 124 011
Amount: 100 µg
Price: $435.00
Cat. No. 124 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 250 (see remarks) gallery  
IHC-P: not tested yet
IHC-G: 1 : 500 (see remarks) gallery  
ExM: 1 : 250 (see remarks) gallery  
Clarity: 1 : 100 (see remarks)
Clone 108E10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to PDZ-domain of mouse PSD95 (UniProt Id: Q62108)
Reactivity Reacts with: rat (P31016), mouse (Q62108), chicken, human (P78352).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 124-01P
Remarks

IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
IHC: For optimal results, glyoxal fixation (IHC-G) or mild PFA fixation (immersion fixation with 4% PFA for 15 min) according to Klotz et al. 2019 is recommended.
IHC-G: Fixation with 9% glyoxal, 8% acetic acid, in ddH2O, pH 4.2-4.4, according to Konno et al. 2023 is recommended.
ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)
Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al., 2023; PMID: 37221592).

Data sheet 124_011.pdf

References for PSD95 - 124 011

G6PD deficiency triggers dopamine loss and the initiation of Parkinson's disease pathogenesis.
Stykel MG, Siripala SV, Soubeyrand E, Coackley CL, Lu P, Camargo S, Thevasenan S, Figueroa GB, So RWL, Stuart E, Panchal R, et al.
Cell reports (2025) 441: 115178. 124 011 WB; tested species: human,mouse
Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.
Libé-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, Gaspariunaite V, et al.
Neuron (2024) : . 124 011 WB; tested species: human,mouse
SNX17 Mediates Dendritic Spine Maturation via p140Cap.
Cui Q, Liang S, Li H, Guo Y, Lv J, Wang X, Qin P, Xu H, Huang TY, Lu Y, Tian Q, et al.
Molecular neurobiology (2023) : . 124 011 WB; tested species: mouse
Orexin induces the production of an endocannabinoid-derived lysophosphatidic acid eliciting hypothalamic synaptic loss in obesity.
Fernández-Rilo AC, Forte N, Palomba L, Tunisi L, Piscitelli F, Imperatore R, Di Costanzo A, Di Marzo V, Cristino L
Molecular metabolism (2023) : 101713. 124 011 WB, IHC; tested species: mouse
Developmental Inhibitory Changes in the Primary Somatosensory Cortex of the Stargazer Mouse Model of Absence Epilepsy.
Hassan M, Grattan DR, Leitch B
Biomolecules (2023) 131: . 124 011 WB; tested species: mouse
Prkn knockout mice show autistic-like behaviors and aberrant synapse formation.
Huo Y, Lu W, Tian Y, Hou Q, Man HY
iScience (2022) 257: 104573. 124 011 WB; tested species: mouse
Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment.
Dumrongprechachan V, Salisbury RB, Butler L, MacDonald ML, Kozorovitskiy Y
eLife (2022) 11: . 124 011 WB; tested species: mouse
Vesicular Glutamate Release from Feeder-FreehiPSC-Derived Neurons.
Baldassari S, Cervetto C, Amato S, Fruscione F, Balagura G, Pelassa S, Musante I, Iacomino M, Traverso M, Corradi A, Scudieri P, et al.
International journal of molecular sciences (2022) 2318: . 124 011 WB; tested species: human
Long-term potentiation reconstituted with an artificial TARP/PSD-95 complex.
Ravi AS, Zeng M, Chen X, Sandoval G, Diaz-Alonso J, Zhang M, Nicoll RA
Cell reports (2022) 412: 111483. 124 011 WB; tested species: mouse
Defective lipid signalling caused by mutations in PIK3C2B underlies focal epilepsy.
Gozzelino L, Kochlamazashvili G, Baldassari S, Mackintosh AI, Licchetta L, Iovino E, Liu YC, Bennett CA, Bennett MF, Damiano JA, Zsurka G, et al.
Brain : a journal of neurology (2022) 1457: 2313-2331. 124 011 WB, IHC; tested species: mouse
Synapsin Condensates Recruit alpha-Synuclein.
Hoffmann C, Sansevrino R, Morabito G, Logan C, Vabulas RM, Ulusoy A, Ganzella M, Milovanovic D
Journal of molecular biology (2021) 43312: 166961. 124 011 WB; tested species: rat
SREBP2 gene therapy targeting striatal astrocytes ameliorates Huntington's disease phenotypes.
Birolini G, Verlengia G, Talpo F, Maniezzi C, Zentilin L, Giacca M, Conforti P, Cordiglieri C, Caccia C, Leoni V, Taroni F, et al.
Brain : a journal of neurology (2021) 14410: 3175-3190. 124 011 WB; tested species: mouse
SIRT-1 Activity Sustains Cholesterol Synthesis in the Brain.
Paulazo MA, Sodero AO
Neuroscience (2021) 476: 116-124. 124 011 WB; tested species: mouse
High-content image-based analysis and proteomic profiling identifies Tau phosphorylation inhibitors in a human iPSC-derived glutamatergic neuronal model of tauopathy.
Cheng C, Reis SA, Adams ET, Fass DM, Angus SP, Stuhlmiller TJ, Richardson J, Olafson H, Wang ET, Patnaik D, Beauchamp RL, et al.
Scientific reports (2021) 111: 17029. 124 011 WB; tested species: mouse
Activation of the medial preoptic area (MPOA) ameliorates loss of maternal behavior in a Shank2 mouse model for autism.
Grabrucker S, Pagano J, Schweizer J, Urrutia-Ruiz C, Schön M, Thome K, Ehret G, Grabrucker AM, Zhang R, Hengerer B, Bockmann J, et al.
The EMBO journal (2021) : e104267. 124 011 WB; tested species: mouse
Vesicle Tethering on the Surface of Phase-Separated Active Zone Condensates.
Wu X, Ganzella M, Zhou J, Zhu S, Jahn R, Zhang M
Molecular cell (2020) : . 124 011 WB
Caspr2 interacts with type 1 inositol 1,4,5-trisphosphate receptor in the developing cerebellum and regulates Purkinje cell morphology.
Argent L, Winter F, Prickett I, Carrasquero-Ordaz M, Olsen AL, Kramer H, Lancaster E, Becker EBE
The Journal of biological chemistry (2020) 29536: 12716-12726. 124 011 WB; tested species: mouse
Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum.
Zhang RS, Liakath-Ali K, Südhof TC
eLife (2020) 9: . 124 011 WB; tested species: mouse
Kir6.1 Heterozygous Mice Exhibit Aberrant Amygdala-Dependent Cued Fear Memory.
Inagaki R, Moriguchi S, Fukunaga K
Molecular neurobiology (2019) : . 124 011 WB; tested species: mouse
Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.
Lazarevic V, Mantas I, Flais I, Svenningsson P
International journal of molecular sciences (2019) 2017: . 124 011 WB; tested species: rat
Rac1 activation links tau hyperphosphorylation and Aβ dysmetabolism in Alzheimer's disease.
Borin M, Saraceno C, Catania M, Lorenzetto E, Pontelli V, Paterlini A, Fostinelli S, Avesani A, Di Fede G, Zanusso G, Benussi L, et al.
Acta neuropathologica communications (2018) 61: 61. 124 011 WB; tested species: mouse
Neogenin in amygdala for neuronal activity and information processing.
Sun XD, Chen WB, Sun D, Huang J, Li YQ, Pan JX, Wang YN, Zhao K, Dong ZQ, Wang HS, Xiong L, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 124 011 WB; tested species: mouse
Mitochondria-Bound Hexokinase (mt-HK) Activity Differ in Cortical and Hypothalamic Synaptosomes: Differential Role of mt-HK in H2O2 Depuration.
Cavalcanti-de-Albuquerque JP, de Souza Ferreira E, de Carvalho DP, Galina A
Molecular neurobiology (2017) : . 124 011 WB; tested species: rat
Proteomic Analysis of Post-synaptic Density Fractions from Shank3 Mutant Mice Reveals Brain Region Specific Changes Relevant to Autism Spectrum Disorder.
Reim D, Distler U, Halbedl S, Verpelli C, Sala C, Bockmann J, Tenzer S, Boeckers TM, Schmeisser MJ
Frontiers in molecular neuroscience (2017) 10: 26. 124 011 WB
PRRT2 Is a Key Component of the Ca(2+)-Dependent Neurotransmitter Release Machinery.
Valente P, Castroflorio E, Rossi P, Fadda M, Sterlini B, Cervigni RI, Prestigio C, Giovedì S, Onofri F, Mura E, Guarnieri FC, et al.
Cell reports (2016) 151: 117-31. 124 011 WB
Mass spectrometric analysis of synaptosomal membrane preparations for the determination of brain receptors, transporters and channels.
Sialana FJ, Gulyassy P, Májek P, Sjöstedt E, Kis V, Müller AC, Rudashevskaya EL, Mulder J, Bennett KL, Lubec G
Proteomics (2016) 1622: 2911-2920. 124 011 WB
Super-Resolution Microscopy Reveals Presynaptic Localization of the ALS/FTD Related Protein FUS in Hippocampal Neurons.
Schoen M, Reichel JM, Demestre M, Putz S, Deshpande D, Proepper C, Liebau S, Schmeisser MJ, Ludolph AC, Michaelis J, Boeckers TM, et al.
Frontiers in cellular neuroscience (2015) 9: 496. 124 011 WB; tested species: rat
Distribution of SNAP25, VAMP1 and VAMP2 in mature and developing deep cerebellar nuclei after estrogen administration.
Manca P, Mameli O, Caria MA, Torrejón-Escribano B, Blasi J
Neuroscience (2014) 266: 102-15. 124 011 IHC, WB
IκB kinase/nuclear factor κB-dependent insulin-like growth factor 2 (Igf2) expression regulates synapse formation and spine maturation via Igf2 receptor signaling.
Schmeisser MJ, Baumann B, Johannsen S, Vindedal GF, Jensen V, Hvalby ØC, Sprengel R, Seither J, Maqbool A, Magnutzki A, Lattke M, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3216: 5688-703. 124 011 WB; tested species: mouse
Effects of phosphorylation and neuronal activity on the control of synapse formation by synapsin I.
Perlini LE, Botti F, Fornasiero EF, Giannandrea M, Bonanomi D, Amendola M, Naldini L, Benfenati F, Valtorta F
Journal of cell science (2011) 124Pt 21: 3643-53. 124 011 WB
Regulation of Neural Circuit Development by Cadherin-11 Provides Implications for Autism.
Frei JA, Niescier RF, Bridi MS, Durens M, Nestor JE, Kilander MBC, Yuan X, Dykxhoorn DM, Nestor MW, Huang S, Blatt GJ, et al.
eNeuro () 84: . 124 011 WB, ICC; tested species: mouse
Cat. No.: 124 011
Amount: 100 µg
Price: $435.00