Synaptic Systems - gamma-Protocadherin

Cat. No.: 190 103

Amount: 50 µg

Price: $375.00

Cat. No. 190 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 808 to 931 from rat γ-Protocadherin (UniProt Id: I6LBW6)
Reactivity	Reacts with: rat (I6LBW6). Other species not tested yet.
Specificity	Detects different γ-protocadherins since they share the constant cytoplasmic tail. K.O. validated PubMed: 29439167
Data sheet	190_103.pdf

References for gamma-Protocadherin - 190 103

Developmental neuronal origin regulates neocortical map formation.
Lin Y, Zhang XJ, Yang J, Li S, Li L, Lv X, Ma J, Shi SH
Cell reports (2023) 423: 112170. 190 103 WB, IHC; KO verified; tested species: mouse

The Role of Protocadherin γ in Adult Sensory Neurons and Skin Reinnervation.
Long RM, Ong H, Wang WX, Komirishetty P, Areti A, Chandrasekhar A, Larouche M, Lefebvre JL, Zochodne DW
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4349: 8348-8366. 190 103 WB, IHC; KO verified; tested species: mouse

Combinatorial effects of Alpha- and Gamma-Protocadherins on neuronal survival and dendritic self-avoidance.
Ing-Esteves S, Kostadinov D, Marocha J, Sing AD, Joseph KS, Laboulaye M, Sanes JR, Lefebvre JL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 190 103 WB; KO verified; tested species: mouse

Phosphorylation of protocadherin proteins by the receptor tyrosine kinase Ret.
Schalm SS, Ballif BA, Buchanan SM, Phillips GR, Maniatis T
Proceedings of the National Academy of Sciences of the United States of America (2010) 10731: 13894-9. 190 103 WB; tested species: mouse

Cat. No.: 190 103

Amount: 50 µg

Price: $375.00

ICC

IHC

Background

Cadherins are a complex protein superfamily involved in many cellular processes including cell recognition, cell signaling, cell communication during embryogenesis, and the formation of neural circuits in the central nervous system. Protocadherins constitute the largest group within the cadherin superfamily and can be subdivided into three groups: α-, β- and γ-protocadherins. Genes for these subgroups are organized in closely related gene clusters and encode variable extracellular and transmembrane domains. The short cytosolic tails are constant and shared within one subgroup.

1 ) Bonn S et al., Mol. Cell. Biol. (2007)
2 ) Cronin KD et al., Int. J. Biol. Sci. (2006)
3 ) Triana-Baltzer GB et al., J. Neurobiol. (2006)
4 ) Taguchi Y et al., Mol. Biol. Evol. (2005)
5 ) Wang X et al., Genes Dev. (2002)
6 ) Obata S et al., J. Cell. Sci. (1995)

Protocols

ICC

IHC

gamma-Protocadherin antibody - 190 103 K.O.

References for gamma-Protocadherin - 190 103