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Home > Products > 308 111

Birch Profilin antibody - 308 111

Profilins are regulators of the microfilament network
Mouse monoclonal purified IgG
Cat. No.: 308 111
Amount: 100 µg
Price: $415.00
Cat. No. 308 111 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 10000
IP: yes
ICC: yes
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 4A6
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 140 from bovine Profilin1 (UniProt Id: P02584)
Epitop AA 42 to 47 from bovine Profilin1 (UniProt Id: P02584)
Specificity Specific for birch profilin.
Remarks

This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).
Data sheet 308_111.pdf

References for Birch Profilin - 308 111

Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody.
Wiedemann P, Giehl K, Almo SC, Fedorov AA, Girvin M, Steinberger P, Rüdiger M, Ortner M, Sippl M, Dolecek C, Kraft D, et al.
The Journal of biological chemistry (1996) 27147: 29915-21. 308 111
Cat. No.: 308 111
Amount: 100 µg
Price: $415.00
Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody.
Wiedemann P, Giehl K, Almo SC, Fedorov AA, Girvin M, Steinberger P, Rüdiger M, Ortner M, Sippl M, Dolecek C, Kraft D, et al.
The Journal of biological chemistry (1996) 27147: 29915-21. 308 111
Background

Profilins are small proteins (14-17 kDa) which are involved in the regulation of the cellular microfilament system. They are associated with highly dynamic microfilament structures present at cellular membranes. Profilins have been found together with lamellipodia, focal adhesions, surface ruffles and on intracellular vesicles and have been shown to interact with different cytoskeleton proteins like actin, gephyrin and the Arp 2/3 complex. Recently a tumor suppression activity has been described for profilin.