Synaptic Systems

Cat. No.: 282 002

Amount: 200 µl

Price: $355.00

Cat. No. 282 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 571 to 766 from mouse PRG1 (UniProt Id: Q7TME0)
Reactivity	Reacts with: rat (Q7TMB7), mouse (Q7TME0). Other species not tested yet.
Data sheet	282_002.pdf

References for PRG1 - 282 002

PRG-1 prevents neonatal stimuli-induced persistent hyperalgesia and memory dysfunction via NSF/Glu/GluR2 signaling.
Liu X, Li S, Zhang W, Xie Z, He J, Zhang X, Yu S, Cao S, Yu T, Xiao Z
iScience (2022) 259: 104989. 282 002 WB, IP, IHC; tested species: rat

Cat. No.: 282 002

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

Plasticity related gene 1 (PRG 1), also referred to as Lppr4, is a brain-specific membrane protein with lipid phosphatase activity that is involved in axonal outgrowth and neuron sprouting.
It has also been shown to interact with calmodulin, a multifunctional Ca2+-signal transducer.

1 ) Trimbuch T et al., Cell (2009)
2 ) Tokumitsu H et al., Biochem. J. (2010)
3 ) Bräuer AU et al., Nat. Neurosci. (2003)

Protocols

Standard protocol

ICC

IHC

PRG1 antibody - 282 002

References for PRG1 - 282 002