Synaptic Systems

Cat. No.: 142 002

Amount: 200 µl

Price: $355.00

Cat. No. 142 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (ECL detection) (see remarks) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: yes gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 4439 to 4776 from rat Piccolo (UniProt Id: Q9JKS6)
Reactivity	Reacts with: rat (Q9JKS6), mouse (Q9QYX7). Other species not tested yet.
Specificity	K.O. validated PubMed: 27537483
Matching control protein/peptide	142-0P
Remarks	WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen. This antibody detects an additional band of ~65 kDa.
Data sheet	142_002.pdf

References for Piccolo - 142 002

ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse

Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 142 002 WB; KO verified; tested species: rat

Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.
Carlson SS, Valdez G, Sanes JR
Journal of neurochemistry (2010) 1153: 654-66. 142 002 WB; tested species: mouse

APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.
Szodorai A, Kuan YH, Hunzelmann S, Engel U, Sakane A, Sasaki T, Takai Y, Kirsch J, Müller U, Beyreuther K, Brady S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2946: 14534-44. 142 002 WB

Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC

The metabolite p-cresol impairs dendritic development, synaptogenesis, and synapse function in hippocampal neurons: Implications for autism spectrum disorder.
Guzmán-Salas S, Weber A, Malci A, Lin X, Herrera-Molina R, Cerpa W, Dorador C, Signorelli J, Zamorano P
Journal of neurochemistry (2022) 1614: 335-349. 142 002 ICC; tested species: rat

Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 142 002 ICC; tested species: mouse

Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 142 002 ICC; tested species: mouse

No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 142 002 ICC, IHC; tested species: human,mouse

Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.
Aparicio GI, Formoso K, León A, Frasch AC, Scorticati C
Frontiers in synaptic neuroscience (2020) 12: 28. 142 002 ICC; tested species: rat

Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 142 002 ICC; tested species: rat

Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 142 002 ICC

Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 142 002 ICC; KO verified

Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release.
Matz J, Gilyan A, Kolar A, McCarvill T, Krueger SR
Proceedings of the National Academy of Sciences of the United States of America (2010) 10719: 8836-41. 142 002 ICC; tested species: rat

Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions.
Tokoro T, Higa S, Deguchi-Tawarada M, Inoue E, Kitajima I, Ohtsuka T
Neuroreport (2007) 184: 313-6. 142 002 ICC

Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment.
Dresbach T, Torres V, Wittenmayer N, Altrock WD, Zamorano P, Zuschratter W, Nawrotzki R, Ziv NE, Garner CC, Gundelfinger ED
The Journal of biological chemistry (2006) 2819: 6038-47. 142 002 ICC

Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification.
Juranek J, Mukherjee K, Rickmann M, Martens H, Calka J, Südhof TC, Jahn R
The European journal of neuroscience (2006) 2411: 3043-52. 142 002 ICC

Molecular profiling reveals synaptic release machinery in Merkel cells.
Haeberle H, Fujiwara M, Chuang J, Medina MM, Panditrao MV, Bechstedt S, Howard J, Lumpkin EA
Proceedings of the National Academy of Sciences of the United States of America (2004) 10140: 14503-8. 142 002 ICC

Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 142 002 IHC; tested species: rat

Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation.
Oprişoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG
Cell reports (2019) 295: 1082-1098.e10. 142 002 IHC; tested species: mouse

Multiple roles of integrin-α3 at the neuromuscular junction.
Ross JA, Webster RG, Lechertier T, Reynolds LE, Turmaine M, Bencze M, Jamshidi Y, Cetin H, Muntoni F, Beeson D, Hodilvala-Dilke K, et al.
Journal of cell science (2017) 13010: 1772-1784. 142 002 IHC; tested species: mouse

Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 142 002 IHC; tested species: mouse

Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones.
Chen J, Billings SE, Nishimune H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 312: 512-25. 142 002 IHC; tested species: mouse

Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys.
Ribic A, Zhang M, Schlumbohm C, Mätz-Rensing K, Uchanska-Ziegler B, Flügge G, Zhang W, Walter L, Fuchs E
Cellular and molecular neurobiology (2010) 306: 827-39. 142 002 IHC

Cat. No.: 142 002

Amount: 200 µl

Price: $355.00

ICC

IHC

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn²⁺ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

1 ) Shapira M et al., Neuron (2003)
2 ) Fenster SD et al., J. Biol. Chem. (2003)
3 ) Fenster SD et al., Int. J. Dev. Neurosci. ()
4 ) Dick O et al., J. Comp. Neurol. (2001)
5 ) Gerber SH et al., EMBO J. (2001)
6 ) Fenster SD et al., Neuron (2000)
7 ) Cases-Langhoff C et al., Eur. J. Cell Biol. (1996)

Protocols

ICC

IHC

OTHER

Preabsorbtion

Other products for this target

Piccolo antibody - 142 002 K.O.

References for Piccolo - 142 002