Peripherin1 antibody - 424 004

Peripherin1 is a class-III neuronal intermediate filament protein

Guinea pig polyclonal antiserum

Cat. No.: 424 004

Amount: 100 µl

Price: $365.00

Cat. No. 424 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P: 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 468 from rat Peripherin (UniProt Id: P21807)
Reactivity	Reacts with: rat (P21807), mouse (P15331). Other species not tested yet.
Remarks	IHC: Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended.
Data sheet	424_004.pdf

Cat. No.: 424 004

Amount: 100 µl

Price: $365.00

IHC

IHC-P

Background

Peripherin is a class-III neuronal intermediate filament protein. It was originally found in neurons of the peripheral nervous system but is also expressed in distinct subpopulations of neurons and nerve fibres throughout the central nervous system.

1 ) Hol EM et al., Cold Spring Harb Perspect Biol (2017)
2 ) Maison S et al., eNeuro ()
3 ) Gorham JD et al., Brain Res. Dev. Brain Res. (1990)
4 ) Wong J et al., J. Neurosci. Res. (1990)

Protocols

IHC

IHC-P