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Home > Products > 109 002

Proton ATPase antibody 116 kDa subunit - 109 002 K.D.

VOa1 is the largest subunit of the vacuolar proton pump in synaptic vesicles and coated vesicles
Rabbit polyclonal antiserum
Cat. No.: 109 002
Amount: 200 µl
Price: $355.00
Cat. No. 109 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 (see remarks) gallery  
IHC: not recommended
IHC-P: 1 : 200 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 826 to 838 from rat Proton ATPase (UniProt Id: P25286)
Reactivity Reacts with: rat (P25286), mouse (Q9Z1G4), hamster.
Other species not tested yet.
Specificity Specific for the α1 116kDa subunit. K.D. validated PubMed: 21795392
Matching control protein/peptide 109-0P
Remarks

WB:  Proton ATPase aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: Methanol fixation is recommended.
Data sheet 109_002.pdf

References for Proton ATPase - 109 002

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 109 002 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 109 002 WB; tested species: rat
BAX inhibitor-1-associated V-ATPase glycosylation enhances collagen degradation in pulmonary fibrosis.
Lee MR, Lee GH, Lee HY, Kim DS, Chung MJ, Lee YC, Kim HR, Chae HJ
Cell death & disease (2014) 5: e1113. 109 002 WB; tested species: human
Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice.
Yao J, de la Iglesia HO, Bajjalieh SM
PloS one (2013) 87: e68215. 109 002 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 109 002 WB; tested species: mouse
A role for presenilins in autophagy revisited: normal acidification of lysosomes in cells lacking PSEN1 and PSEN2.
Zhang X, Garbett K, Veeraraghavalu K, Wilburn B, Gilmore R, Mirnics K, Sisodia SS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3225: 8633-48. 109 002 WB
Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.
Saw NM, Kang SY, Parsaud L, Han GA, Jiang T, Grzegorczyk K, Surkont M, Sun-Wada GH, Wada Y, Li L, Sugita S, et al.
Molecular biology of the cell (2011) 2218: 3394-409. 109 002 WB; KD verified; tested species: rat
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, et al.
Cell (2010) 1417: 1146-58. 109 002 WB, ICC; tested species: mouse
Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 109 002 WB; tested species: mouse
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 109 002 WB; tested species: rat
Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
Seong E, Wainer BH, Hughes ED, Saunders TL, Burmeister M, Faundez V
Molecular biology of the cell (2005) 161: 128-40. 109 002 WB; tested species: mouse
Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation.
Bohnert S, Schiavo G
The Journal of biological chemistry (2005) 28051: 42336-44. 109 002 WB, ICC; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 109 002 ICC; tested species: rat
Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection.
Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, et al.
PLoS pathogens (2014) 102: e1003928. 109 002 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 109 002 ICC; tested species: mouse,rat
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, et al.
Cell (2010) 1417: 1146-58. 109 002 WB, ICC; tested species: mouse
Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids.
Astarie-Dequeker C, Le Guyader L, Malaga W, Seaphanh FK, Chalut C, Lopez A, Guilhot C
PLoS pathogens (2009) 52: e1000289. 109 002 ICC; tested species: human
Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation.
Bohnert S, Schiavo G
The Journal of biological chemistry (2005) 28051: 42336-44. 109 002 WB, ICC; tested species: rat
Cat. No.: 109 002
Amount: 200 µl
Price: $355.00
Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 109 002 WB; tested species: mouse
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 109 002 WB; tested species: rat
BAX inhibitor-1-associated V-ATPase glycosylation enhances collagen degradation in pulmonary fibrosis.
Lee MR, Lee GH, Lee HY, Kim DS, Chung MJ, Lee YC, Kim HR, Chae HJ
Cell death & disease (2014) 5: e1113. 109 002 WB; tested species: human
Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice.
Yao J, de la Iglesia HO, Bajjalieh SM
PloS one (2013) 87: e68215. 109 002 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 109 002 WB; tested species: mouse
A role for presenilins in autophagy revisited: normal acidification of lysosomes in cells lacking PSEN1 and PSEN2.
Zhang X, Garbett K, Veeraraghavalu K, Wilburn B, Gilmore R, Mirnics K, Sisodia SS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3225: 8633-48. 109 002 WB
Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.
Saw NM, Kang SY, Parsaud L, Han GA, Jiang T, Grzegorczyk K, Surkont M, Sun-Wada GH, Wada Y, Li L, Sugita S, et al.
Molecular biology of the cell (2011) 2218: 3394-409. 109 002 WB; KD verified; tested species: rat
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, et al.
Cell (2010) 1417: 1146-58. 109 002 WB, ICC; tested species: mouse
Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2.
Yelamanchili SV, Pendyala G, Brunk I, Darna M, Albrecht U, Ahnert-Hilger G
The Journal of biological chemistry (2006) 28123: 15671-9. 109 002 WB; tested species: mouse
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 109 002 WB; tested species: rat
Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain.
Seong E, Wainer BH, Hughes ED, Saunders TL, Burmeister M, Faundez V
Molecular biology of the cell (2005) 161: 128-40. 109 002 WB; tested species: mouse
Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation.
Bohnert S, Schiavo G
The Journal of biological chemistry (2005) 28051: 42336-44. 109 002 WB, ICC; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 109 002 ICC; tested species: rat
Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection.
Gouzy A, Larrouy-Maumus G, Bottai D, Levillain F, Dumas A, Wallach JB, Caire-Brandli I, de Chastellier C, Wu TD, Poincloux R, Brosch R, et al.
PLoS pathogens (2014) 102: e1003928. 109 002 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 109 002 ICC; tested species: mouse,rat
Lysosomal proteolysis and autophagy require presenilin 1 and are disrupted by Alzheimer-related PS1 mutations.
Lee JH, Yu WH, Kumar A, Lee S, Mohan PS, Peterhoff CM, Wolfe DM, Martinez-Vicente M, Massey AC, Sovak G, Uchiyama Y, et al.
Cell (2010) 1417: 1146-58. 109 002 WB, ICC; tested species: mouse
Phthiocerol dimycocerosates of M. tuberculosis participate in macrophage invasion by inducing changes in the organization of plasma membrane lipids.
Astarie-Dequeker C, Le Guyader L, Malaga W, Seaphanh FK, Chalut C, Lopez A, Guilhot C
PLoS pathogens (2009) 52: e1000289. 109 002 ICC; tested species: human
Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation.
Bohnert S, Schiavo G
The Journal of biological chemistry (2005) 28051: 42336-44. 109 002 WB, ICC; tested species: rat
WB
ICC
IHC-P
Background
The Proton ATPase, also referred to as vacuolar proton pump, is involved in the acidification of many intracellular organelles. The pump is composed of more than 10 subunits, of which the 116 kDa subunit is the largest. This subunit has an N-terminal cytoplasmic domain and a C-terminal transmembrane domain with probably 6 transmembrane regions. The 116 kDa subunit is essential for proton pump activity.
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