Otubain1 antibody - 280 003

Otubain1 is a cysteine protease in the ubiquitin pathway

Rabbit polyclonal purified antibody

Cat. No.: 280 003

Amount: 50 µg

Price: $375.00

Cat. No. 280 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 80 from mouse Otubain1 (UniProt Id: Q7TQI3)
Reactivity	Reacts with: rat (B2RYG6), mouse (Q7TQI3). Other species not tested yet.
Data sheet	280_003.pdf

Cat. No.: 280 003

Amount: 50 µg

Price: $375.00

Background

Ubiquitination of proteins is a dynamic and reversible process that regulates protein stability and turnover in eukaryotes. The removal of ubiquitin is carried out by deubiquitinating (DUB) enzymes which can be subdivided into ubiquitin carboxy-terminal hydrolases (UCHs) and ubiquitin-specific proteases (USPs). The ubiquitin thioesterase Otubain 1 belongs to the latter group of proteins.

1 ) Sun XX et al., EMBO J. (2012)
2 ) Ernst R et al., Mol. Cell (2009)
3 ) Edelmann MJ et al., Biochem. J. (2009)
4 ) Soares L et al., Nat. Immunol. (2004)
5 ) Balakirev MY et al., EMBO Rep. (2003)

Protocols