Cat. No.: 178 003
Amount: 50 µg
Price:
$375.00
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| Cat. No. 178 003 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: not tested yet ICC: not tested yet IHC: 1 : 5000 gallery IHC-P: not tested yet iDISCO: yes |
| Immunogen | Synthetic peptide corresponding to AA 181 to 196 from mouse Otoferlin (UniProt Id: Q9ESF1-1) |
| Reactivity |
Reacts with: mouse (Q9ESF1). Other species not tested yet. Antigen identical in rat. |
| Specificity | K.O. validated PubMed: 37248244 |
| Data sheet | 178_003.pdf |
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Otoferlin is a transmembrane protein with six C2 domains which locates to synaptic vesicles. It belongs to the family of ferlin C2 proteins which are implicated in membrane-membrane fusion events.
Otoferlin is expressed in hair cells of the inner ear and in some brain regions. Hair cell ribbon synapses lack the candidate neuronal calcium sensors synaptotagmin 1, synaptotagmin 2 and synaptotagmin 3 and other proteins typical for conventional synapses like synaptophysin.
By analogy of Ca2+ binding, SNARE-binding and because of some aspects of the physiological phenotype of otoferlin-deficient hair cells, otoferlin is suggested to take the role of a Ca2+ sensor of exocytosis in hair cells. Mutations in the otoferlin gene are associated with the recessive prelingual deafness DFNB9.