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Home > Products > 389 004

Orexin A antibody - 389 004 K.O.

Orexins regulate feeding, sleep, arousal, reward seeking and energy homeostasis
Guinea pig polyclonal antiserum
Cat. No.: 389 004
Amount: 100 µl
Price: $365.00
Cat. No. 389 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500      gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 46 to 65 from mouse Orexin (UniProt Id: O55241)
Reactivity Reacts with: human, rat, mouse (O55241).
Other species not tested yet.
Specificity Recognizes Orexin A with only minor cross-reactivity to the unprocessed precursor protein. Does not cross-react to Orexin B. K.O. validated
Data sheet 389_004.pdf

References for Orexin A - 389 004

Orexin neurons play contrasting roles in itch and pain neural processing via projecting to the periaqueductal gray.
Kaneko T, Oura A, Imai Y, Kusumoto-Yoshida I, Kanekura T, Okuno H, Kuwaki T, Kashiwadani H
Communications biology (2024) 71: 290. 389 004 IHC; tested species: mouse
Lesion of NPY Receptor-expressing Neurons in Perifornical Lateral Hypothalamus Attenuates Glucoprivic Feeding.
Choi PP, Wang Q, Brenner LA, Li AJ, Ritter RC, Appleyard SM
Endocrinology (2024) 1655: . 389 004 IHC; tested species: rat
Esr1+ hypothalamic-habenula neurons shape aversive states.
Calvigioni D, Fuzik J, Le Merre P, Slashcheva M, Jung F, Ortiz C, Lentini A, Csillag V, Graziano M, Nikolakopoulou I, Weglage M, et al.
Nature neuroscience (2023) 267: 1245-1255. 389 004 IHC; tested species: mouse
Hypothalamic orexinergic neurons modulate pain and itch in an opposite way: pain relief and itch exacerbation.
Kaneko T, Kuwaki T, Kashiwadani H
The journal of physiological sciences : JPS (2022) 721: 21. 389 004 IHC; tested species: mouse
Orexin (hypocretin) participates in central autonomic regulation during fight-or-flight response.
Kuwaki T
Peptides (2021) 139: 170530. 389 004 IHC; tested species: mouse
Cat. No.: 389 004
Amount: 100 µl
Price: $365.00
Orexin neurons play contrasting roles in itch and pain neural processing via projecting to the periaqueductal gray.
Kaneko T, Oura A, Imai Y, Kusumoto-Yoshida I, Kanekura T, Okuno H, Kuwaki T, Kashiwadani H
Communications biology (2024) 71: 290. 389 004 IHC; tested species: mouse
Lesion of NPY Receptor-expressing Neurons in Perifornical Lateral Hypothalamus Attenuates Glucoprivic Feeding.
Choi PP, Wang Q, Brenner LA, Li AJ, Ritter RC, Appleyard SM
Endocrinology (2024) 1655: . 389 004 IHC; tested species: rat
Esr1+ hypothalamic-habenula neurons shape aversive states.
Calvigioni D, Fuzik J, Le Merre P, Slashcheva M, Jung F, Ortiz C, Lentini A, Csillag V, Graziano M, Nikolakopoulou I, Weglage M, et al.
Nature neuroscience (2023) 267: 1245-1255. 389 004 IHC; tested species: mouse
Hypothalamic orexinergic neurons modulate pain and itch in an opposite way: pain relief and itch exacerbation.
Kaneko T, Kuwaki T, Kashiwadani H
The journal of physiological sciences : JPS (2022) 721: 21. 389 004 IHC; tested species: mouse
Orexin (hypocretin) participates in central autonomic regulation during fight-or-flight response.
Kuwaki T
Peptides (2021) 139: 170530. 389 004 IHC; tested species: mouse
IHC
IHC
IHC-P
Background
Orexins, also referred to as Hypocretins, are produced by specialized neurons in the lateral hypothalamus, which project to many different regions of the brain. Orexin A (33 amino acids) and Orexin B (28 amino acids) are both produced from a single pre-pro-orexin polypeptide by a cascade of enzymatic reactions. They operate via binding to two closely related G protein-coupled receptors called Orexin receptor 1 and 2 (OxR 1 and OxR 2). OxR 1 is selective for Orexin A whereas OxR 2 binds both peptides with similar affinity.
The orexin system is conserved in mammals, and plays a central role in regulating feeding, sleep/wake cycles, arousal, energy expenditure, reward seeking, cognition, and stress responses. Defects in orexin signaling are involved in diet-induced obesity, diabetes, narcolepsy, panic anxiety disorder, depression, and addiction.