GluN2B antibody - 244 115

GluN2A and B are subunits of ionotropic NMDA type glutamate receptors

Guinea pig polyclonal purified antibody

Cat. No.: 244 115

Amount: 50 µg

Price: $460.00

Cat. No. 244 115	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 918 to 1470 from rat GluN2B (UniProt Id: Q00960)
Reactivity	Reacts with: rat (Q00960), mouse (Q01097). Other species not tested yet.
Specificity	Specific for GluN 2B, no cross-reactivity to GluN 2A.
Data sheet	244_115.pdf

References for GluN2B - 244 115

CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Zhu ZA, Li YY, Xu J, Xue H, Feng X, Zhu YC, Xiong ZQ
Cell reports (2023) 4210: 113202. 244 115 WB; tested species: mouse

Cat. No.: 244 115

Amount: 50 µg

Price: $460.00

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Groc L et al., Proc. Natl. Acad. Sci. U.S.A. (2006)
2 ) Nagy GG et al., Eur. J. Neurosci. (2004)
3 ) Petralia RS et al., Eur. J. Neurosci. (2002)
4 ) Sans N et al., J. Neurosci. (2000)
5 ) Conti F et al., Cereb. Cortex (1999)
6 ) Petralia RS et al., J. Neurosci. (1994)
7 ) Monyer H et al., Science (1992)

Protocols

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