Neuroligin2 antibody - 129 204 K.O.

Neuroligins are postsynaptic cell adhesion molecules

Guinea pig polyclonal antiserum

Cat. No.: 129 204

Amount: 100 µl

Price: $365.00

Cat. No. 129 204	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues surrounding AA760 of rat Neuroligin2 (UniProt Id: Q62888)
Reactivity	Reacts with: rat (Q62888), mouse (Q69ZK9). Other species not tested yet.
Specificity	K.O. validated
Data sheet	129_204.pdf

Cat. No.: 129 204

Amount: 100 µl

Price: $365.00

Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

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2 ) Missler M et al., J. Neurochem. (1998)
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4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

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