Synaptic Systems - Neuroligin1

Cat. No.: 129 003

Amount: 50 µg

Price: $375.00

Cat. No. 129 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 46 to 165 from rat Neuroligin1 (UniProt Id: Q62765)
Reactivity	Reacts with: rat (Q62765), mouse (Q99K10). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	129-0P
Data sheet	129_003.pdf

References for Neuroligin1 - 129 003

A delay in vesicle endocytosis by a C-terminal fragment of N-cadherin enhances Aβ synaptotoxicity.
Teng Z, Kartalou GI, Dagar S, Fraering PC, Lessmann V, Gottmann K
Cell death discovery (2023) 91: 444. 129 003 WB; tested species: mouse

Dysregulation of AMPA Receptor Trafficking and Intracellular Vesicular Sorting in the Prefrontal Cortex of Dopamine Transporter Knock-Out Rats.
Targa G, Mottarlini F, Rizzi B, Leo D, Caffino L, Fumagalli F
Biomolecules (2023) 133: . 129 003 WB; tested species: rat

A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 129 003 WB; tested species: rat

Overexpression of the cell adhesion protein neuroligin-1 induces learning deficits and impairs synaptic plasticity by altering the ratio of excitation to inhibition in the hippocampus.
Dahlhaus R, Hines RM, Eadie BD, Kannangara TS, Hines DJ, Brown CE, Christie BR, El-Husseini A
Hippocampus (2010) 202: 305-22. 129 003 WB

Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 129 003 ICC; tested species: mouse

Cat. No.: 129 003

Amount: 50 µg

Price: $375.00

Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 129 003 ICC; tested species: mouse

Background

Neuroligins form a family of postsynaptic cell surface molecules that interact with β-neurexins. They are 110-120 kDa polypeptides with homology to acetylcholine esterase. Neuroligin1 and neuroligin3 are specifically localized to post-synaptic densities of excitatory synapses whereas neuroligin2 is found exclusively on inhibitory synapses.
Mutations in neuroligin3 and neuroligin4 have been implicated with a rare, heritable form of autism.

1 ) Song JY et al., Proc. Natl. Acad. Sci. U.S.A. (1999)
2 ) Missler M et al., J. Neurochem. (1998)
3 ) Ichtchenko K et al., J. Biol. Chem. (1996)
4 ) Ichtchenko K et al., Cell (1995)
5 ) Südhof TC et al., Nature (1995)
6 ) Chubykin AA et al., Neuron (2007)
7 ) Chubykin AA et al., J. Biol. Chem. (2005)
8 ) Varoqueaux F et al., Eur. J. Cell Biol. (2004)
9 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)

Protocols

OTHER

Preabsorbtion

Other products for this target

Neuroligin1 antibody extracellular domain - 129 003 K.O.

References for Neuroligin1 - 129 003