Synaptic Systems - Neurofilament H

Cat. No.: 171 106

Amount: 50 µg

Price: $380.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 171 106	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Neurofilament H (UniProt Id: P19246)
Reactivity	Reacts with: rat (P16884), mouse (P19246). Other species not tested yet.
Data sheet	171_106.pdf

References for Neurofilament H - 171 106

A novel in vitro model for investigating oligodendroglial maturation and myelin deposition under demyelinating and remyelinating conditions: Impact of microglial depletion and repopulation.
Di Pietro AA, Pasquini LA
Molecular and cellular neurosciences (2024) 129: 103937. 171 106 ICC; tested species: rat

Deletion of β-Neurexins in Mice Alters the Distribution of Dense-Core Vesicles in Presynapses of Hippocampal and Cerebellar Neurons.
Ferdos S, Brockhaus J, Missler M, Rohlmann A
Frontiers in neuroanatomy (2021) 15: 757017. 171 106 ICC; tested species: mouse

From Organotypic Mouse Brain Slices to Human Alzheimer's Plasma Biomarkers: A Focus on Nerve Fiber Outgrowth.
Yilmaz SN, Steiner K, Marksteiner J, Faserl K, Villunger M, Sarg B, Humpel C
Biomolecules (2024) 1410: . 171 106 IHC; tested species: mouse

Reduced Retinal Degeneration in an Oxidative Stress Organ Culture Model through an iNOS-Inhibitor.
Mueller-Buehl AM, Tsai T, Hurst J, Theiss C, Peters L, Hofmann L, Herms F, Kuehn S, Schnichels S, Joachim SC
Biology (2021) 105: . 171 106 IHC; tested species: pig

Activation of Apoptosis in a βB1-CTGF Transgenic Mouse Model.
Weiss M, Reinehr S, Mueller-Buehl AM, Doerner JD, Fuchshofer R, Stute G, Dick HB, Joachim SC
International journal of molecular sciences (2021) 224: . 171 106 IHC; tested species: mouse

Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network.
Jongkamonwiwat N, Ramirez MA, Edassery S, Wong ACY, Yu J, Abbott T, Pak K, Ryan AF, Savas JN
Cell reports (2020) 338: 108431. 171 106 IHC; tested species: mouse

Cat. No.: 171 106

Amount: 50 µg

Price: $380.00

ICC

IHC

IHC-P

Background

Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.

1 ) Barry DM et al., Exp. Cell Res. (2007)
2 ) de Jong D et al., J. Neurol. Neurosurg. Psychiatry (2007)
3 ) Ge WW et al., Mol. Cell. Neurosci. (2007)
4 ) Porchet R et al., Neuroreport (2003)
5 ) Hof PR et al., J. Comp. Neurol. (1996)
6 ) Takeda S et al., J. Cell Biol. (1994)
7 ) Deng Y et al., FASEB J. (2008)
8 ) Fernandes KJ et al., J. Comp. Neurol. (1999)
9 ) Eaker EY et al., Gastroenterology (1990)
10 ) Liem RK et al., J. Cell Biol. (1978)

Protocols

ICC

IHC

IHC-P

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Neurofilament H antibody - 171 106

References for Neurofilament H - 171 106