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Neurobeachin antibody - 194 004

Neurobeachin is a protein kinase A-anchoring protein
Guinea pig polyclonal antiserum
Cat. No.: 194 004
Amount: 100 µl
Price: $365.00
Cat. No. 194 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: yes
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 952 to 1320 from mouse Neurobeachin (UniProt Id: Q9EPN1)
Reactivity Reacts with: rat, mouse (Q9EPN1).
Other species not tested yet.
Remarks

WB: Due to its large size, Neurobeachin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 194_004.pdf
Cat. No.: 194 004
Amount: 100 µl
Price: $365.00
Background
Neurobeachin, also referred to as NBEA, is a large ~330 kDa brain-specific protein that belongs to the BEACH (beige and Chediak-Higashi) family. The protein has been shown to locate to tubulovesicular neuronal endomembranes near the trans-Golgi synapses, but also throughout the cell. Neurobeachin binds to protein kinase A and after adding GTPγS concentrates at Golgi-near membranes.