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Home > Products > 194 003

Neurobeachin antibody - 194 003

Neurobeachin is a protein kinase A-anchoring protein
Rabbit polyclonal purified antibody
Cat. No.: 194 003
Amount: 50 µg
Price: $375.00
Cat. No. 194 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: yes
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 952 to 1320 from mouse Neurobeachin (UniProt Id: Q9EPN1)
Reactivity Reacts with: rat, mouse (Q9EPN1).
Other species not tested yet.
Remarks

WB: Due to its large size, Neurobeachin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet 194_003.pdf

References for Neurobeachin - 194 003

The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation.
Won S, Incontro S, Li Y, Nicoll RA, Roche KW
Proceedings of the National Academy of Sciences of the United States of America (2019) : . 194 003 WB; tested species: mouse
Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
Cat. No.: 194 003
Amount: 50 µg
Price: $375.00
The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation.
Won S, Incontro S, Li Y, Nicoll RA, Roche KW
Proceedings of the National Academy of Sciences of the United States of America (2019) : . 194 003 WB; tested species: mouse
Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Neurobeachin Regulates Glutamate- and GABA-Receptor Targeting to Synapses via Distinct Pathways.
Farzana F, Zalm R, Chen N, Li KW, Grant SG, Smit AB, Toonen RF, Verhage M
Molecular neurobiology (2016) 534: 2112-23. 194 003 WB, ICC
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 194 003 WB, ICC
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
Lauks J, Klemmer P, Farzana F, Karupothula R, Zalm R, Cooke NE, Li KW, Smit AB, Toonen R, Verhage M
PloS one (2012) 76: e39420. 194 003 WB, IP, ICC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
Dendritic spine formation and synaptic function require neurobeachin.
Niesmann K, Breuer D, Brockhaus J, Born G, Wolff I, Reissner C, Kilimann MW, Rohlmann A, Missler M
Nature communications (2011) 2: 557. 194 003 WB, ICC, IHC
WB
ICC
Background
Neurobeachin, also referred to as NBEA, is a large ~330 kDa brain-specific protein that belongs to the BEACH (beige and Chediak-Higashi) family. The protein has been shown to locate to tubulovesicular neuronal endomembranes near the trans-Golgi synapses, but also throughout the cell. Neurobeachin binds to protein kinase A and after adding GTPγS concentrates at Golgi-near membranes.
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