Synaptic Systems - Neurexin1/2/3

Cat. No.: 175 003

Amount: 50 µg

Price: $380.00

Cat. No. 175 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not recommended IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1459 to 1514 and 1657 to 1712 and 1524 to 1578 from rat Neurexin1/2/3
Reactivity	Reacts with: rat (Q63372, Q63376, Q07310), mouse (Q9CS84, E9PUM9, Q8C985). Other species not tested yet.
Specificity	Due to the homology of the cytoplasmic tails of α- and β-neurexins 1, 2 and 3, this antiserum detects all isoforms and their corresponding splice-variants. K.O. validated PubMed: 30104341
Remarks	WB: Non-boiled samples yield stronger signals.
Data sheet	175_003.pdf

References for Neurexin1/2/3 - 175 003

Tandem Mass Tag LC-MS/MS of Aqueous Humor From Individuals With Type 2 Diabetes Without Retinopathy Reveals Early Dysregulation of Synaptic Proteins.
Sachdeva MM, Lee Y, Unlu EK, Koseoglu ND, Cha E, Wang J, Prescott CR, Eghrari AO, Na CH
Investigative ophthalmology & visual science (2024) 653: 16. 175 003 WB; tested species: human

A delay in vesicle endocytosis by a C-terminal fragment of N-cadherin enhances Aβ synaptotoxicity.
Teng Z, Kartalou GI, Dagar S, Fraering PC, Lessmann V, Gottmann K
Cell death discovery (2023) 91: 444. 175 003 WB; tested species: mouse

Altered expression of synaptic proteins and adhesion molecules in the hippocampus and cortex following the onset of diabetes in nonobese diabetic mice.
Yokokawa T, Kido K, Sato K, Hayashi T, Fujita S
Physiological reports (2023) 118: e15673. 175 003 WB; tested species: mouse

SIPA1L1/SPAR1 interacts with the neurabin family of proteins and is involved in GPCR signaling.
Matsuura K, Kobayashi S, Konno K, Yamasaki M, Horiuchi T, Senda T, Hayashi T, Satoh K, Arima-Yoshida F, Iwasaki K, Negishi L, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 175 003 WB; tested species: mouse

Selective expression of the neurexin substrate for presenilin in the adult forebrain causes deficits in associative memory and presynaptic plasticity.
Sánchez-Hidalgo AC, Arias-Aragón F, Romero-Barragán MT, Martín-Cuevas C, Delgado-García JM, Martinez-Mir A, Scholl FG
Experimental neurology (2021) 347: 113896. 175 003 WB; tested species: mouse

α-Neurexins Together with α2δ-1 Auxiliary Subunits Regulate Ca2+ Influx through Cav2.1 Channels.
Brockhaus J, Schreitmüller M, Repetto D, Klatt O, Reissner C, Elmslie K, Heine M, Missler M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3838: 8277-8294. 175 003 WB; KO verified; tested species: mouse

A novel synaptic junction preparation for the identification and characterization of cleft proteins.
Burch A, Tao-Cheng JH, Dosemeci A
PloS one (2017) 123: e0174895. 175 003 WB, EM; tested species: rat

Physical Interactions and Functional Relationships of Neuroligin 2 and Midbrain Serotonin Transporters.
Ye R, Quinlan MA, Iwamoto H, Wu HH, Green NH, Jetter CS, McMahon DG, Veestra-VanderWeele J, Levitt P, Blakely RD
Frontiers in synaptic neuroscience (2015) 7: 20. 175 003 WB; tested species: mouse

Processing of the synaptic cell adhesion molecule neurexin-3beta by Alzheimer disease alpha- and gamma-secretases.
Bot N, Schweizer C, Ben Halima S, Fraering PC
The Journal of biological chemistry (2011) 2864: 2762-73. 175 003 WB

Synapse formation regulated by protein tyrosine phosphatase receptor T through interaction with cell adhesion molecules and Fyn.
Lim SH, Kwon SK, Lee MK, Moon J, Jeong DG, Park E, Kim SJ, Park BC, Lee SC, Ryu SE, Yu DY, et al.
The EMBO journal (2009) 2822: 3564-78. 175 003 WB

Neuroligin-1-Modified Electrodes for Specific Coupling with a Presynaptic Neuronal Membrane.
Jeon J, Yoon SH, Oh MA, Cho W, Kim JY, Shin CI, Kim EJ, Chung TD
ACS applied materials & interfaces (2021) 1318: 21944-21953. 175 003 ICC; tested species: rat

Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3.
Ramirez MA, Ninoyu Y, Miller C, Andrade LR, Edassery S, Bomba-Warczak E, Ortega B, Manor U, Rutherford MA, Friedman RA, Savas JN, et al.
iScience (2022) 258: 104803. 175 003 IHC; tested species: mouse

Diverging Effects of Adolescent Ethanol Exposure on Tripartite Synaptic Development across Prefrontal Cortex Subregions.
Walker CD, Sexton HG, Hyde J, Greene B, Risher ML
Cells (2022) 1119: . 175 003 IHC; tested species: rat

Cat. No.: 175 003

Amount: 50 µg

Price: $380.00

Background

α- and β-neurexins are single pass transmembrane proteins with a short cytoplasmic C-terminus and a long extracellular N-terminal part. In α-neurexins the extracellular sequence is substantially longer than in β-neurexins. Alternative splicing of the N-terminal part even confers more complexity to this protein family suggesting distinct binding partners for the extracellular regions. In contrast, the C-termini are highly conserved in the different isoforms and splice-variants and they share overlapping cytosolic binding partners.
Neurexins are receptor like molecules that form heterologous cell contacts with post-synaptic cell surface proteins at synaptic connections (e.g. β-neurexins with neuroligins). They also serve as receptors for the black widow toxin α-latrotoxin which induces neurotransmitter release.

1 ) Comoletti D et al., Structure (2007)
2 ) Craig AM et al., Curr. Opin. Neurobiol. (2007)
3 ) Chih B et al., Neuron (2006)
4 ) Lisé MF et al., Cell. Mol. Life Sci. (2006)
5 ) Paraoanu LE et al., Neurosci. Lett. (2006)
6 ) Dresbach T et al., Mol. Cell. Neurosci. (2004)
7 ) Comoletti D et al., J. Biol. Chem. (2003)
8 ) Dean C et al., Nat. Neurosci. (2003)
9 ) Tabuchi K et al., Genomics (2002)
10 ) Tobaben S et al., J. Biol. Chem. (2002)

Protocols

Neurexin1/2/3 antibody - 175 003 K.O.

References for Neurexin1/2/3 - 175 003