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Home > Products > 260 011

NET antibody - 260 011

The NE transporter is involved in the termination of synaptic transmission
Mouse monoclonal purified IgG
Cat. No.: 260 011
Amount: 100 µg
Price: $415.00
Cat. No. 260 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 215A9
Subtype IgG1
Immunogen Synthetic peptide corresponding to residues surrounding AA 200 of rat NET. (UniProt Id: Q63380)
Reactivity Reacts with: rat (Q63380), mouse (O55192).
Other species not tested yet.
Data sheet 260_011.pdf

References for NET - 260 011

Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 260 011 IHC; tested species: mouse
Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 260 011 IHC; tested species: mouse
Cat. No.: 260 011
Amount: 100 µg
Price: $415.00
Fluoxetine reverses early-life stress-induced depressive-like behaviors and region-specific alterations of monoamine transporters in female mice.
Zheng JY, Li XX, Liu X, Zhang CC, Sun YX, Ma YN, Wang HL, Su YA, Si TM, Li JT
Pharmacology, biochemistry, and behavior (2024) 237: 173722. 260 011 IHC; tested species: mouse
Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.
Liu X, Sun YX, Zhang CC, Zhang XQ, Zhang Y, Wang T, Ma YN, Wang H, Su YA, Li JT, Si TM, et al.
Neuropharmacology (2021) : 108468. 260 011 IHC; tested species: mouse
IHC
Background
The norepinephrine (NE) transporter (NET), also referred to as Sodium-dependent noradrenaline transporter, is expressed on the plasma membranes of noradrenergic neurons. It is responsible for the inactivation of synaptically released NE and is a target for many antidepressants and psychostimulants.
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