NEDD4-1 antibody - 283 103 K.O.

NEDD4-2 is an ubiquitin ligases involed in cell homeostasis

Rabbit polyclonal purified antibody

Cat. No.: 283 103

Amount: 50 µg

Price: $375.00

Cat. No. 283 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 78 from mouse NEDD4-1 (UniProt Id: P46935)
Reactivity	Reacts with: rat, mouse (Q8CFI0). Other species not tested yet.
Specificity	K.O. validated
Data sheet	283_103.pdf

Cat. No.: 283 103

Amount: 50 µg

Price: $375.00

Background

Nedd 4-1 (RFP 1) and its close relative Nedd 4-2 (NEDD4L) are involved in the ubiquitination of cell surface receptores triggering their internalization and subsequent degradation.
Nedd 4-1 belongs to the most abundant E3 ubiquitin ligases in mammalian neurons. One important target for Nedd 4-1 is Rap2A a protein involved in dendrite formation.
One important target for Nedd 4-2 is the amiloride-sensitive epithelial sodium channel (ENaC) which is involved in fluid and electrolyte homeostasis.

1 ) Boase NA et al., Nat Commun (2011)
2 ) Raikwar NS et al., Am. J. Physiol. Renal Physiol. (2008)
3 ) Wiemuth D et al., Biochem. J. (2007)
4 ) Flores SY et al., Pflugers Arch. (2003)
5 ) Konstas AA et al., J. Biol. Chem. (2002)
6 ) Debonneville C et al., EMBO J. (2001)
7 ) Kamynina E et al., FASEB J. (2001)

Protocols