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NEDD4-2 antibody - 283 003 K.O.

NEDD4-2 is an ubiquitin ligases involed in cell homeostasis
Rabbit polyclonal purified antibody
Cat. No.: 283 003
Amount: 50 µg
Price: $375.00
Cat. No. 283 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Recombinant protein corresponding to AA 256 to 414 from mouse NEDD4-2 (UniProt Id: Q8CFI0)
Reactivity Reacts with: rat (Q62940), mouse (P46935).
Other species not tested yet.
Specificity K.O. validated
Data sheet 283_003.pdf
Cat. No.: 283 003
Amount: 50 µg
Price: $375.00
Background
Nedd 4-1 (RFP 1) and its close relative Nedd 4-2 (NEDD4L) are involved in the ubiquitination of cell surface receptores triggering their internalization and subsequent degradation.
Nedd 4-1 belongs to the most abundant E3 ubiquitin ligases in mammalian neurons. One important target for Nedd 4-1 is Rap2A a protein involved in dendrite formation.
One important target for Nedd 4-2 is the amiloride-sensitive epithelial sodium channel (ENaC) which is involved in fluid and electrolyte homeostasis.