NDCBE antibody - 275 003 K.O.

NDCBE is a sodium bicarbonate exchanger

Rabbit polyclonal purified antibody

Cat. No.: 275 003

Amount: 50 µg

Price: $375.00

Cat. No. 275 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: yes gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 1078 to 1089 from mouse NDCBE (UniProt Id: Q8JZR6)
Reactivity	Reacts with: rat (Q6RVG2), mouse (Q8JZR6). Other species not tested yet.
Specificity	K.O. validated
Remarks	WB: NDCBE aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	275_003.pdf

Cat. No.: 275 003

Amount: 50 µg

Price: $375.00

IHC

Background

The Na⁺-driven Cl^-/HCO3^- exchanger or NDCBE, also known as SLC4A8, is a multispanning transmembrane protein that is predominantly expressed in brain. It is involved in the pH regulation in neurons and modulates glutamatergic synaptic vesicle release.

1 ) Sinning A et al., J. Neurosci. (2011)
2 ) Leviel F et al., J. Clin. Invest. (2010)
3 ) Parker MD et al., Physiol. Genomics (2008)

Protocols

IHC